The Saccharomyces cerevisiae ESU1 gene, which is responsible for enhancement of termination suppression, corresponds to the 3'-terminal half of GAL11.
The Saccharomyces cerevisiae ESU1 gene, which is responsible for enhancement of termination suppression, corresponds to the 3'-terminal half of GAL11.
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酿酒酵母 ESU1 基因负责增强终止抑制，对应于 GAL11 的 3 末端一半。
DOI：
10.1002/yea.1281
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发表时间：
2005
期刊：
影响因子：
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通讯作者：
Chernoff,YuryO
中科院分区：
文献类型：
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作者：
Ono,Bun-ichiro;Futase,Tetsuro;Honda,Waka;Yoshida,Ryohma;Nakano,Kiyohiro;Yamamoto,Takayuki;Nakajima,Erina;Noskov,VladimirN;Negishi,Kazuo;Chen,Buxin;Chernoff,YuryO
从酿酒酵母基因组文库中分离到一个增强[PSI+]依赖性终止抑制因子效率的DNA片段sup 111，其功能归因于1272 bp的ORF。该ORF命名为ESU 1（Enhancer of TerminationSuppression），对应于GAL 11的3′-末端部分。与ESU 1相比，GAL 11对[PSI+] sup 111的抑制效率较低。ESU 1具有TATA‐样序列，其后面有3个ATG密码子，与GAL 11的阅读框架相同，相距约70 bp。通过使用抗Gal 11抗血清的蛋白质印迹分析检测大小对应于预测的Esu 1蛋白的52.7 kDa蛋白。因此，我们得出结论，ESU 1是编码Gal 11 C末端424个氨基酸的多肽的基因。进一步发现ESU 1增加了GAL 11 mRNA的水平，可能也增加了其自身mRNA的水平。此外，ESU 1增加了从leu 2 - 1UAA突变基因转录的mRNA的细胞水平，而GAL 11没有。基于这些发现，我们提出了在用携带ESU 1的质粒转化的[PSI+] sup 111细胞中发生的事件的以下方案：（a）ESU 1刺激leu 2 - 1的转录;（B）leu 2 - 1 mRNA由于具有sup 111（属于upf组）而没有被有效降解;（c）[PSI+]由于eRF 3的耗尽而导致增加的误终止;（d）ESU 1刺激leu 2 - 1的转录;（e）ESU 1刺激leu 2 - 1的转录。（d）使用leu 2 - 1 mRNA产生功能性Leu 2产物;（d）最终实现对leu 2 - 1的抑制。版权所有© 2005年约翰威利父子有限公司。
A DNA fragment enhancing efficiency of [PSI+]‐dependent termination suppressor,sup111, was isolated from a genomic library ofSaccharomyces cerevisiaeand its function was attributed to an ORF of 1272 bp. This ORF, designatedESU1(enhancer of terminationsuppression), corresponded to the 3′‐terminal portion ofGAL11. Contrasting toESU1, GAL11lowered the suppression efficiency of [PSI+]sup111.ESU1possesses a TATA‐like sequence of its own and three ATG codons following it within a distance of about 70 bp and all in the same reading frame asGAL11. A 52.7 kDa protein corresponding in size to the predicted Esu1 protein is detected by western blot analysis using anti‐Gal11 antiserum. We therefore conclude thatESU1is the gene that encodes a polypeptide corresponding to the C‐terminal 424 amino acids of Gal11. It was further found thatESU1increases the level ofGAL11mRNA and probably also of its own mRNA. Moreover,ESU1increased the cellular level of mRNA transcribed from theleu2‐1UAAmutant gene, whileGAL11did not. Based on these findings, we propose the following scheme for the events taking place in the [PSI+]sup111cell that is transformed with anESU1‐bearing plasmid: (a)ESU1stimulates transcription ofleu2‐1; (b)leu2‐1mRNA is not effectively degraded because of the possession ofsup111, which belongs to theupfgroup; (c) [PSI+] causes increased mis‐termination due to depletion of eRF3; (d) functional Leu2 product is made usingleu2‐1mRNA; and (d) suppression ofleu2‐1is eventually accomplished. Copyright © 2005 John Wiley & Sons, Ltd.