Serial observations of colonic carcinogenesis in the rat. Premalignant mucosa binds Ulex europeus agglutinin.
Serial observations of colonic carcinogenesis in the rat. Premalignant mucosa binds Ulex europeus agglutinin.
复制标题
大鼠结肠癌发生的系列观察。
DOI：
10.1016/0016-5085(87)90833-x
复制
发表时间：
1987
期刊：
影响因子：
29.4
通讯作者：
Ahnen,DJ
中科院分区：
文献类型：
--
作者：
Shioda,Y;Brown,WR;Ahnen,DJ
我们评估了某些组织化学测试在结肠癌发生的二甲肼模型中检测癌前粘膜的能力。每两周对对照大鼠和二甲肼治疗大鼠进行降结肠结肠镜活检，持续 29 周。采集脾曲、直肠和任何可见肿瘤的活检标本。标本用高碘酸希夫染色以检测中性粘蛋白，用高铁二胺阿新蓝染色以检测唾液酸化和硫酸化粘蛋白，荧光花生凝集素和荧光紫荆素凝集素。前三项测试均未一致地检测到癌前粘膜。然而，在整个研究过程中，仅与 3% 的对照活检标本结合的 Ulex 欧洲凝集素，与二甲基肼处理的动物中的活检标本数量不断增加，在 13-16 周时最高达到 90% 的阳性率。此外，欧洲荆豆凝集素与来自肿瘤邻近组织的所有活检标本以及 93% 的肿瘤均强烈结合。与对照动物相比，二甲肼处理动物的直肠（但不是脾曲）标本中粘膜萎缩和局灶性发育不良更常见，但组织化学标记物与萎缩或发育不良之间没有相关性。我们得出的结论是，杜鹃花凝集素结合是二甲肼治疗的大鼠癌前结肠粘膜的一致特征。
We evaluated certain histochemical tests for their ability to detect premalignant mucosa in the dimethylhydrazine model of colonic carcinogenesis. Biweekly colonoscopic biopsies of the descending colon were performed for 29 wk in control and dimethylhydrazine-treated rats. Biopsy specimens of the splenic flexure, rectum, and any visualized tumors were taken. The specimens were stained with periodic acid-Schiff to detect neutral mucins, high-iron diamine alcian blue to detect sialylated and sulfated mucins, fluoresceinated peanut agglutinin, and fluoresceinatedUlex europeusagglutinin. None of the first three tests consistently detected premalignant mucosa. However,Ulex europeusagglutinin, which bound to only 3% of control biopsy specimens throughout the course of the study, bound to increasing numbers of biopsy specimens in the dimethylhydrazine-treated animals, reaching a maximum of 90% positivity by 13–16 wk. Moreover,Ulex europeusagglutinin bound strongly to all biopsy specimens from tissues adjacent to tumors and to 93% of tumors. Mucosal atrophy and focal dysplasia were present more frequently in specimens taken from the rectum (but not the splenic flexure) of dimethylhydrazine-treated animals than of control animals, but there was no correlation between the histochemical markers and either atrophy or dysplasia. We conclude thatUlex europeusagglutinin binding is a consistent feature of premalignant colonic mucosa in dimethylhydrazine-treated rats.