[Effect of electroacupuncture intervention combined with polysaccharide of Gastrodia elata Blume on expression of nestin and stem cell factor around the ischemic locus of frontal lobe cortex in local cerebral ischemia rats].
[Effect of electroacupuncture intervention combined with polysaccharide of Gastrodia elata Blume on expression of nestin and stem cell factor around the ischemic locus of frontal lobe cortex in local cerebral ischemia rats].
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发表时间：
2015-04
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通讯作者：
Jian Zhao;Rui Huang;Huai-bin Li;K. Xiong
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作者：
Jian Zhao;Rui Huang;Huai-bin Li;K. Xiong
目的 观察电针（EA）、天麻多糖（PGB）、EA+PGB对脑缺血（CI）大鼠缺血部位周围额叶皮层巢蛋白（Nestin）和干细胞因子（SCF）表达的影响，探讨其改善CI的机制。方法40只成年Sprague-Dawley大鼠随机分为正常对照组、CI模型、EA干预组、PGB干预组和EA+PGB组（每组8只）。 CI模型采用大脑中动脉闭塞法制备。 EA（2 Hz，2 V）应用于“百会”（GV 20）并留下“足三里”（ST 36）30分钟，每天一次，持续14天。 PGB组和EA+PGB组大鼠采用PGB胃饲法，剂量为100mg/kg，每日1次，连续14天。免疫组化检测额叶缺血位点周围额叶中Nestin和SCF的表达。结果与正常对照组比较，模型组大脑皮质局部Nestin、SCF蛋白表达水平显着升高（P < 0.05）。治疗后，与模型组比较，EA组、PGB组和EA+PGB组Nestin和SCF的表达水平进一步显着上调（P < 0.05）。结论 电针联合PGB可显着上调脑缺血大鼠缺血部位周围额叶Nestin和SCF的表达，可能有助于其改善脑缺血的作用。
OBJECTIVE To observe the effect of electroacupuncture (EA), Polysaccharide of Gastrodia elata Blume (PGB), and EA + PGB on the expression of Nestin and stem cell factor (SCF) in the frontal lobe cortex around the ischemic loci of cerebral ischemia (CI) rats, so as to explore its mechanisms underlying improvement of CI. METHODS A total of 40 Sprague-Dawley adult rats were randomly divided into normal control, CI model, EA intervention, PGB intervention and EA + PGB groups (n = 8 in each group). The CI model was prepared by middle cerebral artery occlusion. EA (2 Hz, 2 V) was applied to "Baihui" (GV 20) and left "Zusanli" (ST 36) for 30 min, once daily for 14 days. Rats of the PGB and EA + PGB groups were treated by gastrogavage of PGB at a dose of 100 mg/kg, once daily for 14 successive days. The expression of Nestin and SCF in the frontal lobe around the ischemic loci of the frontal lobe was detected by immunohistochemistry. RESULTS Compared with the normal control group, the expression levels of regional cerebral cortical Nestin and SCF proteins were significantly increased in the model group (P < 0.05). After the treatment, the expression levels of Nestin and SCF were significantly further up-regulated in the EA, PGB and EA + PGB groups in comparison with the model group (P < 0.05). CONCLUSION EA combined with PGB can significantly up-regulate the expression of Nestin and SCF in the frontal lobe around the ischemic loci in cerebral ischemia rats, which may contribute to their function in improving CI.