Interleukin-7 Is Associated with Clinical and Pathological Activities in Immunoglobulin A Nephropathy and Protects the Renal Proximal Tubule Epithelium from Cellular Fibrosis
Interleukin-7 Is Associated with Clinical and Pathological Activities in Immunoglobulin A Nephropathy and Protects the Renal Proximal Tubule Epithelium from Cellular Fibrosis
复制标题
Interleukin-7 与免疫球蛋白 A 肾病的临床和病理活动相关，并保护肾近端小管上皮免受细胞纤维化
DOI：
10.1007/s11596-021-2409-z
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发表时间：
2021-10-01
影响因子：
2.4
通讯作者：
Han, Min
中科院分区：
文献类型：
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作者：
Deng, Yuan-jun;Lin, Xue-ping;Han, Min
目的 免疫球蛋白 A 肾病 (IgAN) 的诊断需要对肾活检标本进行评估。然而，肾活检是一种侵入性检查，由于各种原因并不经常进行。因此，迫切需要公认的非侵入性生物标志物来预测 IgAN 进展。方法本研究纳入2015年6月至2016年5月期间接受肾活检的86例IgAN患者，采用ELISA法测定血浆白细胞介素7（IL-7）水平。分析血浆IL-7水平与临床病理特征之间的关联。采用免疫组织化学染色法检测体内IL-7的原位表达。 Western blotting检测肾小管上皮细胞在TGF-β1刺激后IL-7处理下细胞外基质、p-mTOR和自噬标志物的产生。结果 与健康受试者相比，IgAN 患者的 IL-7 显着降低（2.3077 vs. 8.6294 pg/mL，P
Objective Diagnosis of immunoglobulin A nephropathy (IgAN) requires the evaluation of renal biopsy specimens. However, renal biopsy is an invasive procedure and is not frequently performed for various reasons. Thus, recognized noninvasive biomarkers for predicting IgAN progression are urgently needed. Methods In the present study, we included 86 IgAN patients with renal biopsy from June 2015 to May 2016 and had their plasma interleukin-7 (IL-7) level measured with ELISA. The association between the plasma IL-7 level and clinico-pathological characteristics was analyzed. Immunohistochemical staining was used to assay the in situ expression of IL-7 in vivo. Western blotting was performed to examine the production of extracellular matrix, p-mTOR and the markers of autophagy under the treatment of IL-7 after TGF-beta 1 stimulation in renal tubular epithelial cells. Results IL-7 was significantly decreased in patients with IgAN compared to healthy subjects (2.3077 vs. 8.6294 pg/mL, P