New fluorescent calcium indicators designed for cytosolic retention or measuring calcium near membranes.
New fluorescent calcium indicators designed for cytosolic retention or measuring calcium near membranes.
复制标题
新型荧光钙指示剂，设计用于胞质保留或测量膜附近的钙。
DOI：
10.1016/s0006-3495(95)80082-2
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Poenie,M
中科院分区：
文献类型：
--
作者：
Vorndran,C;Minta,A;Poenie,M
一种新的荧光钙指示剂家族已经基于一种新的BAPTA类似物FF 6开发出来。这种新的BAPTA类似物作为一种通用的合成中间体，用于开发针对特定细胞内环境的Ca 2+指示剂。本报告描述了其中两种新的钙离子指示剂fura-PE 3和fura-FFP 18。Fura-PE 3是一种两性离子指示剂，可抵抗Fura-2和其他聚羧酸钙指示剂所见的快速泄漏和区室化。与用Fura-2获得的结果相反，用PE 3加载的细胞保持明亮的加载并且对细胞溶质游离钙浓度的变化响应数小时。Fura-FFP 18是一种两亲性指示剂，可与脂质体和细胞膜结合。稍后将详细描述的研究表明，FFP 18作为近膜Ca 2+指示剂发挥作用，并且质膜附近的钙水平比胞质溶胶中上升得更快和更高。
A new family of fluorescent calcium indicators has been developed based on a new analog of BAPTA called FF6. This new BAPTA analog serves as a versatile synthetic intermediate for developing Ca2+ indicators targeted to specific intracellular environments. Two of these new Ca2+ indicators, fura-PE3 and fura-FFP18, are described in this report. Fura-PE3 is a zwitterionic indicator that resists the rapid leakage and compartmentalization seen with fura-2 and other polycarboxylate calcium indicators. In contrast to results obtained with fura-2, cells loaded with PE3 remain brightly loaded and responsive to changes in concentration of cytosolic free calcium for hours. Fura-FFP18 is an amphipathic indicator that to binds to liposomes and to cell membranes. Studies to be detailed later indicate that FFP18 functions as a near-membrane Ca2+ indicator and that calcium levels near the plasma membrane rise faster and higher than in the cytosol.