Production of H2O2 by bovine blood and milk polymorphonuclear leucocytes.
Production of H2O2 by bovine blood and milk polymorphonuclear leucocytes.
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牛血和乳汁多形核白细胞产生 H2O2。
DOI：
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
B. Reiter
中科院分区：
文献类型：
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作者：
H. Korhonen;B. Reiter
本文研究了牛多形核白细胞（PMN）释放H_2O_2的能力。静止的PMN悬浮在缓冲液中只释放少量的H2 O2，这是明显增加在吞噬热杀死大肠菌群。然而，在牛血清（BS），胎牛血清（FCS）和乳清（MW）的存在下，没有增加过氧化氢可以检测到，除非叠氮化钠（NaN 2）加入。看来，这些液体的酶含量（过氧化氢酶和乳过氧化物酶）消耗释放的H2 O2和NaN 2，使这些酶失活，消除了这种干扰。活生物体需要BS或MW用于吞噬作用和H2 O2产生。牛IgG 2和IgG 1（在较小程度上）刺激了H2 O2的释放，但SIgA或IgM没有刺激，而与吞噬作用无关;这表明细胞表面存在对IgG 2和IgG 1特异的受体。酪蛋白胶束的摄入引发了H2 O2生产的最大爆发悬浮在缓冲液中的细胞。一般来说，从血液中分离的PMN比从乳汁中分离的细胞更活跃。由于H2 O2的胞外释放反映了H2 O2的胞内水平，因此乳汁PMN较低的代谢活性可能导致乳汁白细胞较低的胞内杀菌活性。过氧化氢的释放可能会激活胞外的乳过氧化物酶系统，已知是在牛奶中杀菌的可能性，进行了讨论。
The ability of bovine polymorphonuclear leucocytes (PMN) to release H2O2 was investigated. Resting PMN suspended in buffer released only small amounts of H2O2 which was appreciably increased during phagocytosis of heat killed coliforms. However, in the presence of bovine serum (BS), foetal calf serum (FCS) and milk whey (MW) no increase of H2O2 could be detected unless sodium azide (NaN2) was added. It appears that the enzyme content of these fluids (catalase and lactoperoxidase) consumed the released H2O2 and NaN2, which inactivates these enzymes, abolished this interference. Live organisms required BS or MW both for phagocytosis and for H2O2 production. Bovine IgG2 and, to a lesser extent, IgG1 but not SIgA or IgM stimulated the release of H2O2 independently of phagocytosis; this indicates the presence of receptors specific for IgG2 and IgG1 on the cell surface. Ingestion of casein micelles triggered the greatest burst of H2O2 production by cells suspended in buffer. In general, PMN isolated from blood were more active than cells isolated from milk. Since the extracellular release of H2O2 reflects the intracellular level of H2O2, the lower metabolic activity of milk PMN may contribute to the lesser intracellular bactericidal activity of milk leucocytes. The possibility that the release of H2O2 may activate extracellularly the lactoperoxidase system, known to be bactericidal in milk, is discussed.