KAINIC ACID SELECTIVELY ALTERS AUDITORY DENDRITES CONNECTED WITH COCHLEAR INNER HAIR-CELLS
KAINIC ACID SELECTIVELY ALTERS AUDITORY DENDRITES CONNECTED WITH COCHLEAR INNER HAIR-CELLS
复制标题
DOI：
10.1016/0378-5955(85)90006-1
复制
发表时间：
1985-01-01
期刊：
影响因子：
2.8
通讯作者：
JOHNSTONE, BM
中科院分区：
文献类型：
--
作者：
PUJOL, R;LENOIR, M;JOHNSTONE, BM
通过圆窗向成年豚鼠和大鼠以及6日龄幼鼠的耳蜗注射2 μ l含有1 nmol红藻氨酸（KA）的人工经典小西外淋巴液。5分钟后，将它们固定、取出并处理用于电子显微镜检查。在所有KA处理的耳蜗中，注射导致内毛细胞（IHC）下方的听觉树突严重肿胀。在外毛细胞（OHC）下方，肿胀仅出现在6日龄大鼠中，而不在成年动物中。这些结果在三个方面具有重要意义：（1）证实了成人耳蜗内毛细胞和外毛细胞的传入神经有很大差异。(2)他们揭示了突触发生过程中OHC水平的突触可塑性。(3)他们支持谷氨酸或相关物质是IHC β-递质的假设。
Cochleas of adult guinea pigs and rats, and 6-day-old rat pups, were injected, through the round window, with 2 .mu.l of artificial classical Konishi perilymph containing 1 nmol kainic acid (KA). 5 min later, they were fixed, removed, and processed for electron microscopy. In all KA-treated cochleas, the injection resulted in a severe swelling of auditory dendrites below the inner hair cells (IHCs). Below the outer hair cells (OHCs), the swelling appeared only in the 6-day-old rats, not in adult animals. These results are significant in three different ways: (1) They confirm the strong difference between afferents innervating the IHCs and the OHCs in adult cochleas. (2) They shed some light on the synaptic plasticity found at the OHC level during synaptogenesis. (3) They support the hypothesis that glutamate, or a related substance, is the IHC neutrotransmitter.