Bacterial origin of a diverse family of UDP-glycosyltransferase genes in the Tetranychus urticae genome
Bacterial origin of a diverse family of UDP-glycosyltransferase genes in the Tetranychus urticae genome
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DOI：
10.1016/j.ibmb.2014.04.003
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发表时间：
2014-07-01
影响因子：
3.8
通讯作者：
Van Leeuwen, Thomas
中科院分区：
文献类型：
--
作者：
Ahn, Seung-Joon;Dermauw, Wannes;Van Leeuwen, Thomas
UDP-糖基转移酶（UGT）催化各种小的亲脂性分子与尿苷二磷酸（UDP）糖的结合，将其转化为更具水溶性的代谢产物。因此，UGT在外源性物质的解毒和内源性物质的调节中发挥重要作用。最近，二斑叶螨的基因组序列被报道，二斑叶螨是一种多食性草食动物，危害许多农作物。虽然在T.荨麻疹，UGT至今没有。我们确定了80个UGT基因的荨麻疹基因组中，最大数量的UGT基因在一个后生动物物种的报道。系统发育分析表明，谱系特异性基因扩增增加了荨麻疹UGT库的多样性。对T.还描述了荨麻疹UGT。此外，表达谱后，寄主植物的转变和杀螨剂抗性线支持UGT基因在异源生物质代谢的重要作用。在其他蛛形纲动物（螯肢亚门）中对UGT的扩展搜索，包括蜘蛛，蝎子，两种蜱和两种捕食螨，意外地发现完全不存在UGT基因。然而，蜈蚣（多足亚门）和水蚤和小龙虾（甲壳亚门）在其基因组中含有类似于昆虫UGT的UGT基因，这表明UGT基因家族可能在Chelicerata谱系的早期丢失，随后在叶螨中重新获得。T.二斑叶螨UGT和细菌UGT及其系统发育重建结果表明，叶螨通过水平基因转移从细菌获得了UGT基因。我们的研究结果显示了一个独特的进化历史的T urticae UGT基因家族在其他节肢动物中，并提供了重要的线索，其功能有关的解毒，从而主机适应。(C)2014爱思唯尔有限公司版权所有。
UDP-glycosyltransferases (UGTs) catalyze the conjugation of a variety of small lipophilic molecules with uridine diphosphate (UDP) sugars, altering them into more water-soluble metabolites. Thereby, UGTs play an important role in the detoxification of xenobiotics and in the regulation of endobiotics. Recently, the genome sequence was reported for the two-spotted spider mite, Tetranychus urticae, a polyphagous herbivore damaging a number of agricultural crops. Although various gene families implicated in xenobiotic metabolism have been documented in T. urticae, UGTs so far have not. We identified 80 UGT genes in the T urticae genome, the largest number of UGT genes in a metazoan species reported so far. Phylogenetic analysis revealed that lineage-specific gene expansions increased the diversity of the T urticae UGT repertoire. Genomic distribution, intron-exon structure and structural motifs in the T. urticae UGTs were also described. In addition, expression profiling after host-plant shifts and in acaricide resistant lines supported an important role for UGT genes in xenobiotic metabolism. Expanded searches of UGTs in other arachnid species (Subphylum Chelicerata), including a spider, a scorpion, two ticks and two predatory mites, unexpectedly revealed the complete absence of UGT genes. However, a centipede (Subphylum Myriapoda) and a water flea and a crayfish (Subphylum Crustacea) contain UGT genes in their genomes similar to insect UGTs, suggesting that the UGT gene family might have been lost early in the Chelicerata lineage and subsequently re-gained in the tetranychid mites. Sequence similarity of T. urticae UGTs and bacterial UGTs and their phylogenetic reconstruction suggest that spider mites acquired UGT genes from bacteria by horizontal gene transfer. Our findings show a unique evolutionary history of the T urticae UGT gene family among other arthropods and provide important clues to its functions in relation to detoxification and thereby host adaptation. (C) 2014 Elsevier Ltd. All rights reserved.