Active suppression of leaflet emergence as a mechanism of simple leaf development
Active suppression of leaflet emergence as a mechanism of simple leaf development
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DOI：
10.1038/s41477-021-00965-3
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发表时间：
2021-07-26
期刊：
影响因子：
18
通讯作者：
Nath, Utpal
中科院分区：
文献类型：
--
作者：
Challa, Krishna Reddy;Rath, Monalisha;Nath, Utpal
被子植物的叶片表现出广泛的形状多样性，大致分为两种形式：具有完整叶片的单叶和具有解剖成小叶的叶片的复叶。边缘解剖和小叶启动的机制主要是通过分析复叶结构来推断的，因此，单叶的完整叶片是否有可能在内源基因失活时启动小叶尚不清楚。在这里，我们证明了CININNAA样teosinte BRANCHED1，CINCLOIDEA，增殖细胞因子(CIN-TCP)转录因子激活了类KNOTTED1(KNOX-II)基因，CIN-TCP和KNOX-II蛋白共同冗余地抑制了单叶的小叶起始。在拟南芥中，CIN-TCP和KNOX-II的同时下调导致茎基因KNOX-I和CUPSHAPED子叶(CUC)的重新激活，并触发异位器官发生，最终将简单的叶片转化为似乎无限启动小叶的超复合形式。因此，一种保守的发育机制促进了简单的叶结构，其中CIN-TCPKNOX-II形成了一个强大的分化模块，抑制了KNOX-I-CUC网络和小叶的启动。单叶有可能成为复叶吗？在拟南芥中，CININNATA样teosinte BRANCHED1，CINCLOIDEA，增殖细胞因子(CIN-TCP)转录因子和类KNOTTED1(KNOX-II)转录因子的联合作用抑制了单叶的小叶起始。这些基因的下调导致了超级复合叶的产生。
Angiosperm leaves show extensive shape diversity and are broadly divided into two forms; simple leaves with intact lamina and compound leaves with lamina dissected into leaflets. The mechanistic basis of margin dissection and leaflet initiation has been inferred primarily by analysing compound-leaf architecture, and thus whether the intact lamina of simple leaves has the potential to initiate leaflets upon endogenous gene inactivation remains unclear. Here, we show that the CINCINNATA-like TEOSINTE BRANCHED1, CYCLOIDEA, PROLIFERATING CELL FACTORS (CIN-TCP) transcription factors activate the class II KNOTTED1-LIKE (KNOX-II) genes and the CIN-TCP and KNOX-II proteins together redundantly suppress leaflet initiation in simple leaves. Simultaneous downregulation of CIN-TCP and KNOX-II in Arabidopsis leads to the reactivation of the stemness genes KNOX-I and CUPSHAPED COTYLEDON (CUC) and triggers ectopic organogenesis, eventually converting the simple lamina to a super-compound form that appears to initiate leaflets indefinitely. Thus, a conserved developmental mechanism promotes simple leaf architecture in which CIN-TCP-KNOX-II forms a strong differentiation module that suppresses the KNOX-I-CUC network and leaflet initiation.Do simple leaves have the potential to become compound leaves? In Arabidopsis, the combined action of CINCINNATA-like TEOSINTE BRANCHED1, CYCLOIDEA, PROLIFERATING CELL FACTORS (CIN-TCP) transcription factors and class II KNOTTED1-LIKE (KNOX-II) transcription factors suppresses leaflet initiation in simple leaves. Downregulation of these genes leads to super-compound leaves.