Analysis of cross-reactivity of five new chicken monoclonal antibodies which recognize the apical complex of Eimeria using confocal laser immunofluorescence assay
Analysis of cross-reactivity of five new chicken monoclonal antibodies which recognize the apical complex of Eimeria using confocal laser immunofluorescence assay
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DOI：
10.1016/j.vetpar.2003.09.011
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发表时间：
2003-12-01
影响因子：
2.6
通讯作者：
Baba, E
中科院分区：
文献类型：
--
作者：
Constantinoiu, CC;Lillehoj, HS;Baba, E
对于顶复合体（成员）来说，寄主细胞的入侵是借助位于寄生物顶端的细胞器实现的。本文描述了5种鸡抗艾美耳球虫孢子虫单克隆抗体的性质。所有单抗均与鸡艾美耳球虫孢子体顶端复合体的分子发生反应。免疫荧光分析(IFA)一个马伯,8 e 1,认出一个顶端提示分子出现在所有鸡艾美球虫属子孢子,两个马伯(8 d2和他)承认抗原出现在顶端提示相同的两个艾美球虫属物种(e . acervulina和大肠brunetti),另一个马伯(5 d-11)公认的抗原出现在顶端的其他两个物种(大肠acervulina和大肠最大值),而一个马伯(8颈- 3)识别抗原存在子孢子和孢囊壁只有大肠acervulina。除了顶端抗原外，HE-4和8D-2两个单抗还识别了位于孢子体前半部分的一些蛋白质。总的来说，这些单抗证明鸡艾美耳球虫孢子体的顶端复合体具有一种或多种抗原，这些抗原有望在宿主细胞识别和入侵中发挥作用。(C) 2003 Elsevier B.V.版权所有
For Apicomplexa (members) the host cell invasion is realized with the help of the organelles located at the apical tip of parasites. In this research paper the characterization of five chicken monoclonal antibodies (mabs) produced against Eimeria acervulina sporozoites is described. All mabs reacted with molecules belonging to the apical complex of chicken Eimeria sporozoites. On immunofluorescence assay (IFA) one mab, 8E-1, recognized an apical tip molecule present on all chicken Eimeria sporozoites, two mabs (8D-2 and HE-4) recognized an antigen present on the apical tip of the same two Eimeria species (E. acervulina and E. brunetti), another mab (5D-11) recognized an antigen present on the apical tip of other two species (E. acervulina and E. maxima) while one mab (8C-3) identified antigens present on the sporozoites and sporocysts wall of only E. acervulina. Besides the apical tip antigens, two mabs (HE-4 and 8D-2) recognized some proteins located in the anterior half of the sporozoites. Collectively, these mabs proved that the apical complex of chicken Eimeria sporozoites share one or more antigens that are expected to play a role in host cell recognition and invasion. (C) 2003 Elsevier B.V. All rights reserved.