Quantitative trait locus analysis of symbiotic nitrogen fixation activity in the model legume Lotus japonicus
Quantitative trait locus analysis of symbiotic nitrogen fixation activity in the model legume Lotus japonicus
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DOI：
10.1007/s10265-011-0459-1
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发表时间：
2011-10
影响因子：
2.8
通讯作者：
Akiyoshi Tominaga;T. Gondo;R. Akashi;Shao-hui Zheng;S. Arima;A. Suzuki
中科院分区：
文献类型：
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作者：
Akiyoshi Tominaga;T. Gondo;R. Akashi;Shao-hui Zheng;S. Arima;A. Suzuki
许多豆科植物形成固氮根瘤。这种豆科植物固氮作用的提高将对植物生长和农业生物量生产产生重大影响。为探明模式豆科植物百脉根固氮调控的遗传基础，以宫古岛MG-20 ×岐阜B-129的重组自交系为材料，对百脉根固氮调控的QTL进行了分析。该群体接种中生根瘤菌lotiMAFF 303099，并在含有蛭石的豆荚中生长14天。收集了单株乙炔还原活性（ARA/P）、单位根瘤重（ARA/NW）、单位根瘤数（ARA/NN）、单株NN、单株NW、茎长（SL）、未接种SL（SLbac−）、未接种地上部干重（SWbac −）、未接种根长（RLbac−）和根干重（RWbac−）的表型数据，共检测到34个QTL。ARA/P、ARA/NN、NW和SL表现出很强的相关性和QTL共定位，表明共生固氮的几个重要植物特征受同一位点控制。ARA/P，ARA/NN，NW和SL的QTL，共定位在第4染色体上的标记TM 0832周围，也与先前报道的种子质量QTL共定位。这是首次报道共生固氮活性性状的QTL分析。
Many legumes form nitrogen-fixing root nodules. An elevation of nitrogen fixation in such legumes would have significant implications for plant growth and biomass production in agriculture. To identify the genetic basis for the regulation of nitrogen fixation, quantitative trait locus (QTL) analysis was conducted with recombinant inbred lines derived from the cross Miyakojima MG-20 × Gifu B-129 in the model legumeLotus japonicus. This population was inoculated withMesorhizobium lotiMAFF303099 and grown for 14 days in pods containing vermiculite. Phenotypic data were collected for acetylene reduction activity (ARA) per plant (ARA/P), ARA per nodule weight (ARA/NW), ARA per nodule number (ARA/NN), NN per plant, NW per plant, stem length (SL), SL without inoculation (SLbac−), shoot dry weight without inoculation (SWbac−), root length without inoculation (RLbac−), and root dry weight (RWbac−), and finally 34 QTLs were identified. ARA/P, ARA/NN, NW, and SL showed strong correlations and QTL co-localization, suggesting that several plant characteristics important for symbiotic nitrogen fixation are controlled by the same locus. QTLs for ARA/P, ARA/NN, NW, and SL, co-localized around marker TM0832 on chromosome 4, were also co-localized with previously reported QTLs for seed mass. This is the first report of QTL analysis for symbiotic nitrogen fixation activity traits.