Prominent 85-kDa oligomannosidic glycoproteins of rat brain are signal regulatory proteins and include the SHP substrate-1 for tyrosine phosphatases.
Prominent 85-kDa oligomannosidic glycoproteins of rat brain are signal regulatory proteins and include the SHP substrate-1 for tyrosine phosphatases.
复制标题
大鼠脑中重要的 85 kDa 寡甘露糖苷糖蛋白是信号调节蛋白，包括酪氨酸磷酸酶的 SHP 底物-1。
DOI：
10.1046/j.1471-4159.1999.721688.x
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发表时间：
1999
影响因子：
4.7
通讯作者：
Quarles,RH
中科院分区：
文献类型：
--
作者：
Bartoszewicz,ZP;Jaffe,H;Sasaki,M;Moller,JR;Stebbins,JW;Gebrekristos,H;Quarles,RH
Abstract:The glycoprotein component in rat brain reacting most strongly withGalanthus nivalisagglutinin (GNA) on western blots migrates as an 85‐kDa band. GNA identifies mannose‐rich oligosaccharides because it is highly specific for terminal α‐mannose residues. After purification of this 85‐kDa glycoprotein band by chromatography on GNA‐agarose and preparative gel electrophoresis, binding of other lectins demonstrated the presence of fucose and a trace of galactose, but no sialic acid. Treatment withN‐Glycanase or endoglycosidase H produced a 65‐kDa band, indicating that it consisted of about one‐fourth N‐linked oligomannosidic carbohydrate moieties. High‐performance anion‐exchange chromatography and fluorescence‐assisted carbohydrate electrophoresis indicated that the major carbohydrate moiety is a heptasaccharide with the structure Manα1‐6(Manα1‐3)Manα1‐6(Manα1‐3)Manβ1‐4Glc‐NAcβ1‐4GlcNAc (Man5GlcNAc2). Determination of amino acid sequences of peptides produced by endoproteinase digestion demonstrated that this 85‐kDa mannose‐rich glycoprotein component contained the SHP substrate‐1 for phosphotyrosine phosphatases and at least one other member of the signal‐regulatory protein (SIRP) family. The unusually high content of oligomannosidic carbohydrate moieties on these receptor‐like members of the immunoglobulin superfamily in neural tissue could be of functional significance for intercellular adhesion or signaling.
