MicroRNA-224 suppresses colorectal cancer cell migration by targeting Cdc42.
MicroRNA-224 suppresses colorectal cancer cell migration by targeting Cdc42.
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DOI：
10.1155/2014/617150
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Cheng CW
中科院分区：
文献类型：
--
作者：
Ke TW;Hsu HL;Wu YH;Chen WT;Cheng YW;Cheng CW
肿瘤细胞的转移扩散是影响结直肠癌患者临床预后的主要危险因素。转移表型可以通过肿瘤细胞不同结构和功能蛋白质的合成失调来调节。微RNA是一类非编码RNA，通过与3′端非翻译区的序列特异性相互作用识别其同源信使RNA靶点，并参与CRC发展的多步骤过程。本研究旨在探讨miR-224在结直肠癌中的表达及其生物学作用。采用实时荧光定量PCR方法检测79例结直肠癌组织和18例非肿瘤组织中miR-224的表达水平。miR-224在结直肠癌组织中的表达水平显著低于癌旁组织。其表达水平与APC基因突变状态有关。miR-224的异位表达抑制了CRC细胞的迁移能力，但对细胞增殖的影响较小。增加miR-224在蛋白和mRNA水平上减少Cdc 42和SMAD 4表达，并抑制肌动蛋白丝的形成。总之，这项研究表明miR-224在负调控CRC细胞迁移中的作用。miR-224的表达水平可能是CRC进展的有用的预测生物标志物。
The metastatic spread of tumor cells is the major risk factor affecting the clinical prognosis of colorectal cancer (CRC) patients. The metastatic phenotype can be modulated by dysregulating the synthesis of different structural and functional proteins of tumor cells. Micro(mi)RNAs are noncoding RNAs that recognize their cognate messenger (m)RNA targets by sequence-specific interactions with the 3′ untranslated region and are involved in the multistep process of CRC development. The objective of this study was to investigate the expression and biological roles of miR-224 in CRC. The miR-224 expression level was assessed by a quantitative real-time PCR in 79 CRC and 18 nontumor tissues. Expression levels of miR-224 in CRC tissues were significantly lower than those in nontumor tissues. Its expression level was associated with the mutation status of the APC gene. Ectopic expression of miR-224 suppressed the migratory ability of CRC cell line, but cell proliferation was less affected. Increased miR-224 diminished Cdc42 and SMAD4 expressions at both the protein and mRNA levels and inhibited the formation of actin filaments. Overall, this study indicated a role of miR-224 in negatively regulating CRC cell migration. The expression level of miR-224 may be a useful predictive biomarker for CRC progression.
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影响因子：
2.8
作者：
Prueitt, Robyn L.;Yi, Ming;Hudson, Robert S.;Wallace, Tiffany A.;Howe, Tiffany M.;Yfantis, Harris G.;Lee, Dong. H.;Stephens, Robert M.;Liu, Chang-Gong;Calin, George A.;Croce, Carlo M.;Ambs, Stefan
通讯作者：
Ambs, Stefan
影响因子：
3.7
作者：
Guo, Chunguang;Sah, Jerome F.;Beard, Lydia;Willson, James K. V.;Markowitz, Sanford D.;Guda, Kishore
通讯作者：
Guda, Kishore
影响因子：
--
作者：
Liu H;Brannon AR;Reddy AR;Alexe G;Seiler MW;Arreola A;Oza JH;Yao M;Juan D;Liou LS;Ganesan S;Levine AJ;Rathmell WK;Bhanot GV
通讯作者：
Bhanot GV
影响因子：
9.3
作者：
Mavridis, Konstantinos;Stravodimos, Konstantinos;Scorilas, Andreas
通讯作者：
Scorilas, Andreas
影响因子：
--
作者：
Lin J;Wang X;Dorsky RI
通讯作者：
Dorsky RI