Parental bias of Ki-ras oncogenes detected in lung tumors from mouse hybrids.
Parental bias of Ki-ras oncogenes detected in lung tumors from mouse hybrids.
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在小鼠杂交小鼠的肺肿瘤中检测到 Ki-ras 致癌基因的亲本偏倚。
DOI：
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发表时间：
1992
影响因子：
11.1
通讯作者：
Marshall W. Anderson
中科院分区：
文献类型：
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作者：
M. You;Yian Wang;G. Stoner;L. You;R. Maronpot;S. Reynolds;Marshall W. Anderson
将肺肿瘤易感性低的小鼠品系（C3 H）和肺肿瘤易感性高的小鼠品系（A/J）进行双杂交，产生C3 A和AC 3 F1杂交小鼠。Ki-ras癌基因在C3 A和AC 3小鼠自发和化学诱导的肺肿瘤中检测到。为了进一步探讨Ki-ras基因在小鼠肺肿瘤易感性中的遗传学，通过基于A/J Ki-ras等位基因的第二内含子中的37个碱基对缺失的策略来确定在来自F1杂种的肺肿瘤中检测到的Ki-ras癌基因的亲本来源。Ki-ras癌基因来自A/J亲本的38/40个肿瘤来自C3 A小鼠和30/30个肿瘤来自AC 3小鼠。杂交体中激活的癌基因来源于易感亲本的观察结果表明Ki-ras基因与小鼠肺肿瘤易感性直接相关。这一发现可能对人类肺腺癌的发展有影响，因为在35%的这种人类肿瘤类型中检测到Ki-ras癌基因。
A mouse strain with low lung tumor susceptibility (C3H) and a strain with high lung tumor susceptibility (A/J) were reciprocally crossed to produce C3A and AC3 F1 hybrid mice. Ki-ras oncogenes were detected in spontaneous and chemically induced lung tumors obtained from the C3A and AC3 mice. To further explore the genetics of the Ki-ras gene in mouse lung tumor susceptibility, the parental origin of Ki-ras oncogenes detected in lung tumors from the F1 hybrids was determined by a strategy based on a 37-base-pair deletion in the second intron of the A/J Ki-ras allele. Ki-ras oncogenes were derived from the A/J parent in 38 of 40 tumors obtained from C3A mice and 30 of 30 tumors from AC3 mice. The observation that the activated oncogene in hybrids originates from the susceptible parent suggests that the Ki-ras gene is directly linked to mouse lung tumor susceptibility. This finding may have implications for pulmonary adenocarcinoma development in humans, since Ki-ras oncogenes are detected in 35% of this human tumor type.