E3, a hematopoietic-specific transcript directly regulated by the retinoic acid receptor alpha
E3, a hematopoietic-specific transcript directly regulated by the retinoic acid receptor alpha
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DOI：
10.1182/blood.v88.7.2517.bloodjournal8872517
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发表时间：
1996-10-01
期刊：
影响因子：
20.3
通讯作者：
Collins, SJ
中科院分区：
文献类型：
--
作者：
Scott, LM;Mueller, L;Collins, SJ
Retinoic acid (RA)-induced maturation mediated by the retinoic acid receptor alpha (RAR alpha) has been implicated in myeloid development. We have used differential hybridization analysis of a cDNA library constructed from the murine RA-induced MPRO promyelocyte cell line to identify immediate-early genes induced by RA during granulocytic differentiation. E3, one of nine sequences identified, was upregulated in an immediate-early manner, with transcript levels peaking after 60 minutes exposure to RA. E3 transcripts were RA-inducible HL60 cells, but not in an RA-resistant subclone, HL60R. that harbors a mutated RAR alpha gene. However. when HL60R cells were transduced with a functional copy of the RAR alpha gene, RA induced a 10-fold increase in E3 mRNA levels. E3 transcripts are present in the myeloid, B-lymphoid, and erythroid lineages. absent in nonhematopoietic cells, and encode a highly hydrophobic, potentially phosphorylated polypeptide of unknown function with significant homology to a putative protein expressed in myeloid cells. The murine E3 promoter harbors a single bipartite retinoic acid response element which in transient transfection assays conferred RA sensitivity. These results indicate that E3 is a hematopoietic-specific gene that is an immediate target for the activated RAR alpha during myelopoiesis. (C) 1996 by The American Society of Hematology.
