Late expression of M and N antigens on glycophorin A during erythroid differentiation
Late expression of M and N antigens on glycophorin A during erythroid differentiation
复制标题
红系分化过程中血型糖蛋白 A 上 M 和 N 抗原的晚期表达
DOI：
10.1182/blood.v66.1.233.233
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发表时间：
1985
期刊：
影响因子：
20.3
通讯作者：
L. Andersson
中科院分区：
文献类型：
--
作者：
M. Ekblom;C. Gahmberg;L. Andersson
M/N血型由主要的人类红细胞唾液糖蛋白糖蛋白a携带，o -糖苷碳水化合物是其活性所必需的，但M/N特异性是由于分子中nh2末端部分的氨基酸替换。我们使用M和N血型特异性单克隆抗体来研究它们在正常红细胞生成过程中的表达。在这里，我们报告了在多色正母细胞阶段之前，M/N血型活性非常弱或根本不表达。利用多克隆抗糖蛋白A抗血清，我们发现糖蛋白A分子已经大量存在于最早形态可识别的红细胞前体，红原细胞上。这些发现可以用我们之前的观察来解释，即在红细胞成熟过程中，糖蛋白A的o -糖基化逐渐增加。
The M/N blood groups are carried by the major human red cell sialoglycoprotein, glycophorin A. O-glyosidic carbohydrate is needed for the activity, but the M/N specificity is due to amino acid replacements in the NH2-terminal portion of the molecule. We have used monoclonal antibodies specific for M and N blood groups to study their expression during normal erythropoiesis. Here we report that the M/N blood group activities are very weakly or not at all expressed before the polychromatic normoblast stage. Using polyclonal anti-glycophorin A antiserum, it was shown that glycophorin A molecules are already abundantly present on the earliest morphologically recognizable erythroid precursor, the proerythroblast. These findings can be explained by our previous observation that the O-glycosylation of glycophorin A gradually increases during erythroid maturation.