The interaction of CpEBF1 with CpMADSs is involved in cell wall degradation during papaya fruit ripening
The interaction of CpEBF1 with CpMADSs is involved in cell wall degradation during papaya fruit ripening
复制标题
CpEBF1 与 CpMADSs 的相互作用参与木瓜果实成熟过程中细胞壁的降解
DOI：
10.1038/s41438-018-0095-1
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发表时间：
2019-01-01
影响因子：
8.7
通讯作者：
Li, Xueping
中科院分区：
文献类型：
--
作者：
Ding, Xiaochun;Zhu, Xiaoyang;Li, Xueping
Ethylene plays a pivotal role in climacteric fruit ripening; whereas 1-MCP, a non-toxic antagonist of ethylene, prevents ethylene-dependent responses and fruit ripening. In this study, a short-term treatment (1 h) with 400 nL L-1 1-MCP delayed the ripening of harvested papaya. However, long-term application of 1-MCP (400 nL L-1, 16 h) resulted in abnormal fruit ripening, with the fruits exhibiting normal yellowing without softening, significantly higher cellulose and lignin contents, and intact cell walls (CW). Furthermore, we found that long-term treatment with 1-MCP significantly inhibited the expression of CpEBF1, an EIN3-binding F-box-1 gene. A protein interaction analysis using yeast two-hybrid, BiFC and GST pull-down assays showed that CpEBF1 interacts with the CpMADS1/3 and CpElL1 proteins. The interaction of CpEBF1 with CpMADS1/3 further activated the activities of CW-degradation gene promoters. Subcellular localization showed that these proteins were localized in the nucleus. Additionally, the expression levels of CpMADS1/3, CpEIL1, and several CW-degradation-related genes were significantly downregulated by long-term 1-MCP treatment. Therefore, we propose that the inhibited expression of CpEBF1 and CpMADS1/3 resulted in the repressed activation of CW-degradation-related genes via their interaction, thereby resulting in fruit softening disorders.
