Muscarinic receptor subtypes in subpopulations of human blood mononuclear cells as analyzed by RT-PCR technique
Muscarinic receptor subtypes in subpopulations of human blood mononuclear cells as analyzed by RT-PCR technique
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DOI：
10.1016/0165-5728(96)00079-3
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发表时间：
1996-08-01
影响因子：
3.3
通讯作者：
Nordberg, A
中科院分区：
文献类型：
--
作者：
HellstromLindahl, E;Nordberg, A
在这项研究中，我们分析了mRNA编码的m1-m5 mAChR亚型在人血单个核细胞和淋巴细胞亚群的表达，采用逆转录-聚合酶链反应（RT-PCR）技术。从人血单个核细胞、T细胞、单核细胞、EB病毒转化的B细胞和两种白血病细胞系中提取总RNA，并通过RT-PCR进行分析。我们的研究结果表明，m3，m4和m5毒蕈碱亚型的mRNA在单核细胞和纯化的T细胞中表达，而在这些细胞中未检测到mi和m2 mRNA。在B细胞和单核细胞中未检测到m1-m5亚型mRNA，表明这些细胞中不存在毒蕈碱受体。白血病T细胞系中毒蕈碱亚型的表达。Peer的细胞学特性，而HL-60细胞系与外周血单个核细胞有明显的差异。Peer细胞中m3和m5亚型均表达，而m4亚型不表达，而HL-60细胞中检测到m4和m5亚型。
In this study we have analysed the expression of mRNA encoding the m1-m5 mAChR subtypes in human blood mononuclear cells and subpopulations of lymphocytes using reverse transcriptase-polymerase chain reaction (RT-PCR) technique. Total RNA was extracted from human blood mononuclear cells, T cells, monocytes, EB virus transformed B cells and from two leukemic cell lines and analysed by RT-PCR. Our results indicate that mRNAs for the m3, m4 and m5 muscarinic subtypes are expressed in mononuclear cells and purified T cells while mi and m2 mRNAs were not detected in these cells. No m1-m5 subtype mRNA was detected in B cells and monocytes, indicating absence of muscarinic receptors in these cells. The expression of muscarinic subtypes in the leukemic T cell line. Peer, and the promyelocytic leukemic cell line HL-60 was different from peripheral mononuclear cells. Both m3 and m5 subtypes were expressed in Peer cells but not the m4 subtype, whereas the m4 and m5 subtypes were detected in HL-60 cells.