Inhibition of the expression of cell-associated fructosyltransferase in Streptococcus salivarius by octyl beta-D-glucopyranoside.
Inhibition of the expression of cell-associated fructosyltransferase in Streptococcus salivarius by octyl beta-D-glucopyranoside.
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辛基β-D-吡喃葡萄糖苷抑制唾液链球菌中细胞相关果糖基转移酶的表达。
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
N. A. Jacques
中科院分区：
文献类型：
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作者：
N. A. Jacques
辛基 β-D-吡喃葡萄糖苷可阻止在分批培养物中生长或在非增殖细胞悬浮培养基中孵育的唾液链球菌 ATCC 25975 中细胞相关果糖基转移酶活性的表达。这种效应既不是由于酶活性的直接抑制，也不是由于活性酶损失到外部介质中。酶表达的阻止似乎并不是由于蛋白质分泌的一般易位机制受到抑制，因为在辛基β-D-吡喃葡萄糖苷存在下生长的裂解细胞的细胞质内未检测到果糖基转移酶活性；也没有观察到细胞外葡萄糖基转移酶的分泌受到任何抑制。这些和其他观察结果支持这样的观点：果糖基转移酶在与细胞表面结合之前并不以活性形式分泌穿过细胞质膜。
Octyl beta-D-glucopyranoside prevented the expression of cell-associated fructosyltransferase activity in Streptococcus salivarius ATCC 25975 grown in batch culture or incubated in nonproliferating cell suspension medium. This effect was not due to the direct inhibition of enzyme activity nor due to the loss of active enzyme into the external medium. The prevention of enzyme expression did not appear to be due to the inhibition of a general translocation mechanism for protein secretion, since fructosyltransferase activity was not detected within the cytoplasm of lysed cells grown in the presence of octyl beta-D-glucopyranoside; nor was there any observed inhibition of the secretion of the extracellular enzyme glucosyltransferase. These and other observations supported the view that fructosyltransferase was not secreted across the cytoplasmic membrane in an active form before becoming associated with the cell surface.