TNF-alpha, PDGF, and TGF-beta(1) expression by primary mouse bronchiolar-alveolar epithelial and mesenchymal cells: tnf-alpha induces TGF-beta(1).
TNF-alpha, PDGF, and TGF-beta(1) expression by primary mouse bronchiolar-alveolar epithelial and mesenchymal cells: tnf-alpha induces TGF-beta(1).
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原代小鼠细支气管肺泡上皮细胞和间充质细胞表达 TNF-α、PDGF 和 TGF-β(1)：TNF-α 诱导 TGF-β(1)。
DOI：
10.1006/exmp.2001.2376
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发表时间：
2001
影响因子：
3.6
通讯作者：
Brody,AR
中科院分区：
文献类型：
--
作者：
Warshamana,GS;Corti,M;Brody,AR
The bronchiolar–alveolar epithelium (BAE) is a primary target site among the multitude of mediators that are released during lung cell injury. 2001 Academic Press for inhaled agents that cause lung injury. These cells, consequently, release a broad range of mediators that influence other cell populations, Key Words: alveolar type II cells; growth factors; RNase protection assay; Northern analysis; immunocytochemistry. including interstitial lung fibroblasts that are central to the development of interstitial pulmonary fibrosis (IPF). A number of peptide growth factors (GF) have been postulated to be essential in the pathogenesis of IPF. We demonstrate here that primary populations of mouse BAE and mesenchymal cells, maintained in culture, synthesize four potent GF. These are platelet-derived growth factor isoforms (PDGF) A and INTRODUCTIONB, transforming growth factor beta-1 (TGF-β1), and tumor necrosis factor alpha (TNF-α). A mouse lung epithelial cell isolation technique pioneered in this laboratory has been used to purify the BAE cells to greater than 85%(80 5.6% alveolar type II and 9 2.3% Clara It has become clear over the past 2 decades that the lung cells) in culture. Northern analysis, RNase protection assay, and immu-
