Regulation of nuclear envelope dynamics via APC/C is necessary for the progression of semi-open mitosis in Schizosaccharomyces japonicus
Regulation of nuclear envelope dynamics via APC/C is necessary for the progression of semi-open mitosis in Schizosaccharomyces japonicus
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DOI：
10.1111/gtc.12072
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发表时间：
2013-09-01
期刊：
影响因子：
2.1
通讯作者：
Niki, Hironori
中科院分区：
文献类型：
--
作者：
Aoki, Keita;Shiwa, Yuh;Niki, Hironori
真核细胞有三种类型的有丝分裂，即开放式、封闭式和半开放式有丝分裂。开放式有丝分裂涉及在核分裂之前破坏核被膜，而封闭式有丝分裂则在完整的核被膜下进行。为了解真核生物3种有丝分裂方式的机制和意义，我们研究了裂殖酵母SchizosophyomycesEscherichia的半开放式有丝分裂过程。在后期促进复合物/细胞周期体（APC/C）突变体的Sz。但是，在分裂后期，核膜保持相对完整，导致半开放有丝分裂受损。作为apc 2突变体的抑制子，获得了3-氧代酰基-[酰基载体蛋白]还原酶Oar 2的突变。APC/C突变体中具有两个APC/C识别的破坏盒基序的Oar 2的水平增加。此外，在apc 2突变体中观察到的有缺陷的半开放有丝分裂被突变的oar 2（+）恢复。基于这些发现，我们建议，APC/C调节动态的核膜通过降解Oar 2依赖于APC/C在中期到后期的过渡半开放有丝分裂在Sz。- 是的
Three types of mitosis, which are open, closed or semi-open mitosis, function in eukaryotic cells, respectively. The open mitosis involves breakage of the nuclear envelope before nuclear division, whereas the closed mitosis proceeds with an intact nuclear envelope. To understand the mechanism and significance of three types of mitotic division in eukaryotes, we investigated the process of semi-open mitosis, in which the nuclear envelope is only partially broken, in the fission yeast Schizosaccharomyces japonicus. In anaphase-promoting complex/cyclosome (APC/C) mutants of Sz. japonicus, the nuclear envelope remained relatively intact during anaphase, resulting in impaired semi-open mitosis. As a suppressor of apc2 mutant, a mutation of Oar2, which was a 3-oxoacyl-[acyl carrier protein] reductase, was obtained. The level of the Oar2, which had two destruction-box motifs recognized by APC/C, was increased in APC/C mutants. Furthermore, the defective semi-open mitosis observed in an apc2 mutant was restored by mutated oar2(+). Based on these findings, we propose that APC/C regulates the dynamics of the nuclear envelope through degradation of Oar2 dependent on APC/C during the metaphase-to-anaphase transition of semi-open mitosis in Sz. japonicus.