Standardization of methods to record Vagus nerve activity in mice.
Standardization of methods to record Vagus nerve activity in mice.
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DOI：
10.1186/s42234-018-0002-y
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发表时间：
2018-01-01
影响因子：
--
通讯作者：
Tracey, Kevin J
中科院分区：
文献类型：
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作者：
Silverman, Harold A;Stiegler, Andrew;Tracey, Kevin J
背景技术背景：迷走神经在器官功能的调节中发挥着重要作用，包括调节免疫和炎症的反射途径。最近使用转基因小鼠的研究提高了我们对免疫神经控制分子机制的理解。然而，映射小鼠迷走神经中传输的神经信号受到技术挑战的限制。在这里，我们已经标准化的实验方案，以记录复合动作电位在迷走神经nerve.METHODS：迷走神经被隔离在Balb/c和B6.129S小鼠，并放置在钩或袖口电极。来自迷走神经的电信号使用Neuralynx或Plexon数据采集系统进行数字化。结果：我们研制了一套电生理记录系统，可记录小鼠颈迷走神经复合动作电位。与钩状电极相比，袖带电极显著降低了背景噪声并提高了信噪比。基线迷走神经活动随麻醉深度和食物摄入而变化。对不同小鼠品系（Balb/c和C57 BL/6）的迷走神经图的分析显示基线活动没有显著差异。重要的是，迷走神经neurogramactivity在野生型和TLR 4受体敲除小鼠表现出内毒素介导的signals.CONCLUSIONS：这些方法记录迷走神经在小鼠中提供了一个有用的工具，以进一步描绘迷走神经通路的作用，在一个标准化的小鼠疾病模型。
BACKGROUND: The vagus nerve plays an important role in the regulation of organ function, including reflex pathways that regulate immunity and inflammation. Recent studies using genetically modified mice have improved our understanding of molecular mechanisms in the neural control of immunity. However, mapping neural signals transmitted in the vagus nerve in mice has been limited by technical challenges. Here, we have standardized an experimental protocol to record compound action potentials transmitted in the vagus nerve.METHODS: The vagus nerve was isolated in Balb/c and B6.129S mice, and placed either on a hook or cuff electrode. The electrical signals from the vagus nerve were digitized using either a Neuralynx or Plexon data acquisition system. Changes in the vagus nerve activity in response to anesthesia, feeding and administration of bacterial endotoxin were analyzed.RESULTS: We have developed an electrophysiological recording system to record compound action potentials from the cervical vagus nerve in mice. Cuff electrodes significantly reduce background noise and increase the signal to noise ratio as compared to hook electrodes. Baseline vagus nerve activity varies in response to anesthesia depth and food intake. Analysis of vagus neurograms in different mouse strains (Balb/c and C57BL/6) reveal no significant differences in baseline activity. Importantly, vagus neurogramactivity in wild type and TLR4 receptor knock out mice exhibits receptor dependency of endotoxin mediated signals.CONCLUSIONS: These methods for recording vagus neurogram in mice provide a useful tool to further delineate the role of vagus neural pathways in a standardized murine disease model.