In vitro behavior of hematopoietic progenitor cells under the influence of chemoattractants: Stromal cell-derived factor-1, steel factor, and the bone marrow environment
In vitro behavior of hematopoietic progenitor cells under the influence of chemoattractants: Stromal cell-derived factor-1, steel factor, and the bone marrow environment
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DOI：
10.1182/blood.v91.1.100.100_100_110
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发表时间：
1998-01-01
期刊：
影响因子：
20.3
通讯作者：
Broxmeyer, HE
中科院分区：
文献类型：
--
作者：
Kim, CH;Broxmeyer, HE
多种化学引诱剂如何协同指导造血祖细胞（HPC）的迁移以归巢和外周血动员尚未确定，我们在这里报告了两种不同的化学引诱剂，基质细胞衍生因子(SDF)-1和钢铁因子（SLF）的影响下HPC的行为，以及骨髓（BM）环境的趋化性质。采用不同的公式来量化这些效应。基于这些定量，SDF-1仅显示趋化活性，而SLF对因子依赖性MO7e细胞显示趋化活性和趋化动力学活性，SLF和SDF-1一样，从CD34（+）细胞群体中吸引人HPC，并诱导MO7e细胞中的肌动蛋白聚合。SLF和SDF-1共同吸引MO7e细胞，以及脐带血（CB）和骨髓CD34（+）细胞。消极的浓度梯度SLF SDF-1,由化学引诱物的存在在参议院,显示有效的抑制影响MO7e细胞迁移引起的这两种化学引诱物在众议院,SDF-1和SLF协同在动员细胞众议院从这个负化学引诱物梯度,等离子体从BM吸入物、但不是CB或外周血,表现出强烈的趋药性的BM和CB CD34(+)细胞的影响,以及对SDF-1依赖性CD34（+）细胞迁移的负梯度抑制作用。这些体外迁移实验表明，SDF-1和SLF等化学引诱剂与其他未识别的BM化学引诱剂可能共同参与HPC向BM的迁移并阻止HPC自发动员出BM。(C) 1998年由美国血液病学会出版。
How multiple chemoattractants cooperate in directing the migration of hematopoietic progenitor cells (HPC) for homing and peripheral blood mobilization has not yet been established, We report here the behavior of HPC under the influence of two different chemoattractants, stromal cell-derived factor (SDF)-1 and steel factor (SLF), and the chemotactic nature of the bone marrow (BM) environment using a two-chamber in vitro migration system. Various formulae were adopted to quantitate these effects. Based on these quantitations, SDF-1 showed only chemotactic activity, while SLF showed both chemotactic and chemokinetic activities on factor-dependent MO7e cells, SLF, like SDF-1, attracted human HPC from a population of CD34(+) cells and induced actin polymerization in MO7e cells. SLF and SDF-1 cooperated in attracting MO7e cells, as well as cord blood (CB) and BM CD34(+) cells. A negative concentration gradient of SLF and SDF-1, formed by the presence of chemoattractants in the upper chamber, showed potent inhibitory effects on MO7e cell migration induced by either of these chemoattractants in the lower chamber, and SDF-1 and SLF were synergistic in mobilizing cells to the lower chamber from this negative chemoattractant gradient, Plasma obtained from BM aspirates, but not CB or peripheral blood, showed strong chemotactic effects on BM and CB CD34(+) cells, and an inhibitory effect in a negative gradient on SDF-1-dependent CD34(+) cell migration, These in vitro migration experiments suggest that chemoattractants such as SDF-1 and SLF with other unidentified BM chemoattractants may be involved cooperatively in the migration of HPC to the BM and in preventing spontaneous mobilization of HPC out of the BM. (C) 1998 by The American Society of Hematology.