Evaluation of the GenoType Mycobacterium assay for identification of mycobacterial species from cultures
Evaluation of the GenoType Mycobacterium assay for identification of mycobacterial species from cultures
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DOI：
10.1128/jcm.44.5.1769-1775.2006
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发表时间：
2006-05-01
影响因子：
9.4
通讯作者：
Hillemann, Doris
中科院分区：
文献类型：
--
作者：
Richter, Elvira;Rusch-Gerdes, Sabine;Hillemann, Doris
一种新的可商业化的DNA条带分析(分枝杆菌CM/AS基因型，Hain生命科学，Nehren.德国)对分枝杆菌物种的区分能力进行了评估。这项测试是基于一种针对23S rRNA基因区域的聚合酶链式反应技术，然后是反向杂交和线探针技术。基因CM能够识别23个物种，该基因作为另外的14个物种，单独或与一个或多个物种不良结合。对156株分枝杆菌菌株进行了评价，其中包括61个有效发表的种，包括不同的亚种，6个非有效发表的种，以及3个除分枝杆菌外的菌株。对所有菌株进行16S rRNA基因5‘端序列测定和生化试验。总体而言，151个菌株的结果是可解释的。在148株分枝杆菌中，CM法与AS法的符合率为92.6%(137/148)，AS法的符合率为89.9%(133/148)，均为非分枝杆菌。
A new commercially available DNA strip assay (GenoType Mycobacterium CM/AS, Hain Lifescience, Nehren.. Germany) was evaluated for the ability to differentiate mycobacterial species. The test is based oil a PCR technique targeting a 23S rRNA gene region, followed by reverse hybridization and line probe technology. The GenoType CM is capable of identifying 23, the GenoType AS a further 14, species either alone or ill combination with one or more species. Both tests were evaluated with 156 mycobacterial strains composed of 61 validly published species including different subspecies, 6 not validly published species, and 3 strains other than mycobacterial species. All strains were precharacterized by sequencing of the 5' region of the 16S rRNA gene and biochemical tests. In total, results for 151 strains were interpretable. Concordant results were obtained for 137 (92.6%) of 148 mycobacterial strains with the CM assay and 133 (89.9%) of 148 mycobacterial strains with the AS assay, and all three non-Mycobacterium species were identified.