Distinct and sequential tissue-specific activities of the LIM-class. homeobox gene Lim1 for tubular morphogenesis during kidney development
Distinct and sequential tissue-specific activities of the LIM-class. homeobox gene Lim1 for tubular morphogenesis during kidney development
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DOI：
10.1242/dev.01858
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发表时间：
2005-06-01
期刊：
影响因子：
4.6
通讯作者：
Behringer, RR
中科院分区：
文献类型：
--
作者：
Kobayashi, A;Kwan, KM;Behringer, RR
肾脏器官发生需要上皮小管的形态发生。分支输尿管芽和后肾间充质之间的诱导相互作用导致间充质到上皮的转变和管状形态发生以形成肾单位，肾的功能单位。LIM类同源异型盒基因Lim1在中间中胚层、肾小管、中肾小管、输尿管芽、前小管聚集体及其衍生物中表达。Lim1基因缺失的小鼠由于肾导管形成失败而缺乏肾脏，这排除了Lim1在肾脏发育后期作用的研究。在这里，我们表明，Lim1功能在不同的组织隔间的发展中的后肾的输尿管芽和肾单位形成的肾泡的图案的适当发展。这些观察结果表明，Lim1在肾脏器官发生过程中的上皮管状形态发生的多个步骤中具有重要作用。我们还表明，肾管是必不可少的延长和维护相邻的苗勒管，原基的女性生殖道。
Kidney organogenesis requires the morphogenesis of epithelial tubules. Inductive interactions between the branching ureteric buds and the metanephric mesenchyme lead to mesenchyme-to-epithelium transitions and tubular morphogenesis to form nephrons, the functional units of the kidney. The LIM-class homeobox gene Lim1 is expressed in the intermediate mesoderm, nephric duct, mesonephric tubules, ureteric bud, pretubular aggregates and their derivatives. Lim1-null mice lack kidneys because of a failure of nephric duct formation, precluding studies of the role of Lim1 at later stages of kidney development. Here, we show that Lim1 functions in distinct tissue compartments of the developing metanephros for both proper development of the ureteric buds and the patterning of renal vesicles for nephron formation. These observations suggest that Lim1 has essential roles in multiple steps of epithelial tubular morphogenesis during kidney organogenesis. We also demonstrate that the nephric duct is essential for the elongation and maintenance of the adjacent Mullerian duct, the anlage of the female reproductive tract.