T lymphocyte regeneration after transplantation of T cell depleted allogeneic bone marrow.
T lymphocyte regeneration after transplantation of T cell depleted allogeneic bone marrow.
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T细胞耗尽的同种异体骨髓移植后T淋巴细胞再生。
DOI：
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发表时间：
1986
影响因子：
4.6
通讯作者：
H. Blacklock
中科院分区：
文献类型：
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作者：
G. Janossy;H. Prentice;J. Grob;K. Ivory;N. Tidman;J. Grundy;M. Favrot;M. Brenner;D. Campana;H. Blacklock
采用免疫荧光双标记法对37例HLA相合的去T淋巴细胞骨髓移植（BMT）治疗血液病患者的T细胞亚群再生进行了研究。抗泛T（CD 6：MBG 6）和抗抑制/细胞毒性T细胞（CD 8：RFT 8）单克隆抗体的混合物与兔血清一起用作溶细胞补体的来源，以实现选择性T细胞裂解。T8+细胞在移植后约60天达到低正常值，并在整个研究期间（超过150天）保持在正常范围内。这一观察结果与我们先前发表的患者结果相反，这些患者在接受BMT后没有有效的T细胞耗竭，从移植后60天开始循环T8+细胞数量增加。在本研究中，Leu-7+，RFT 8-细胞迅速达到正常值，但T4+淋巴细胞的重建缓慢：仅在BMT后150天左右达到低正常水平。移植物抗宿主病（GVHD）的程度似乎与输注的残留T细胞数量有关：接受最高数量T细胞的三名患者中有两名发展为II级和III级;否则GVHD是最小的。在所研究的临床参数中，巨细胞病毒（CMV）免疫状态对重建有中度影响：在移植后55-90天，15例接受CMV血清阴性供体BMT的患者中有7例T8+细胞处于正常上限水平，但16例接受血清阳性供体BMT的患者中无一例T8+细胞处于正常上限水平。CMV相关并发症相对少见。因此，在再生过程中防止“T8+细胞过冲”和T细胞失衡的最重要因素似乎是骨髓中T（包括T8+）淋巴细胞的耗竭。本研究和先前研究中T8+细胞重建的差异可能反映了与接种的成熟T细胞相反的T细胞前体的不同再生模式。
The regeneration of T cell subsets was studied with double immunofluorescence marker methods in 37 patients who received HLA matched T lymphocyte depleted bone marrow transplants (BMT) as part of the treatment for their haematological disease. A cocktail of anti-pan-T (CD6: MBG6) and anti-suppressor/cytotoxic-T cell (CD8: RFT8) monoclonal antibodies was used with rabbit serum as a source of cytolytic complement to achieve selective T cell lysis. The T8+ cells reached low normal values around 60 days post-transplant and remained within the normal range throughout the study (greater than 150 days). This observation is in contrast to our previously published results in patients who, after receiving BMT without efficient T cell depletion, had increased numbers of circulation T8+ cells from 60 days post-transplant. In the present study Leu-7+, RFT8- cells reached normal values rapidly but the reconstitution of T4+ lymphocytes was slow: low normal levels were reached only around day 150 following BMT. The degree of graft-versus-host disease (GVHD) seemed to be related to the number of residual T cells infused: two of the three patients who received the highest numbers of T cells developed Grade II III; otherwise GVHD was minimal. Among the clinical parameters studied cytomegalovirus (CMV) immune status moderately influenced reconstitution: at 55-90 days post-transplant T8+ cells were present at the upper normal levels in seven out of 15 patients receiving BMT from CMV seronegative donors, but in none of the 16 individuals receiving BMT from seropositive donors. CMV related complications were relatively uncommon. Thus the most significant factor in preventing 'T8+ cell overshoot' and T cell imbalance during regeneration appears to be the depletion of T (including T8+) lymphocytes from marrow. The differences of T8+ cell reconstitution in this and previous studies may reflect a different regeneration pattern from T cell precursors as opposed to inoculated mature T cells.