Neutrophil dynamics and retention in lung, oxygenator, and arterial filter during cardiopulmonary bypass in a pig model.
Neutrophil dynamics and retention in lung, oxygenator, and arterial filter during cardiopulmonary bypass in a pig model.
复制标题
猪模型体外循环期间中性粒细胞动态和肺、氧合器和动脉过滤器中的滞留。
DOI：
10.1097/00002480-199407000-00059
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Sfakianakis,GN
中科院分区：
文献类型：
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作者：
Dewanjee,MK;Palatianos,GN;Kapadvanjwala,M;Hsu,LC;Novak,S;Balantino,G;Serafini,AN;Dietrich,WD;Sfakianakis,GN
中性粒细胞与心肺旁路 (CPB) 回路组件中吸附蛋白的相互作用以及活化中性粒细胞上白细胞粘附分子的表达影响中性粒细胞动力学和边缘化。肺和骨骼肌以及体外回路中的氧合器 (OX) 和动脉过滤器 (AF) 提供中性粒细胞 (N) 相互作用的主要区域。在 4 组 20 只约克夏猪（28-35 kg，5 次假手术；5 次 CPB，1 小时；5 次 CPB，3 小时；5 次 CPB，肝素化回路，3 小时）中，使用自体 In-111 标记的中性粒细胞 (INN) 对 3 小时 CPB 期间的 N 相互作用和 N 保留动态进行量化；麻醉猪在 CPB 前 30 分钟注射 INN (500–650 µCi) 并肝素化，并使用滚子泵、中空纤维 OX (Bentley CM 50, 5.0 m 2) 和 AF (Bentley AF 025, 0.25 m 2) 以 2.5–3.6 l/min 进行 CPB 3 小时。 OX 和 AF 上的 N 动力学通过校准的盖革探针进行监测。中性粒细胞沉积，就像 OX 上的血浆蛋白一样，几乎立即达到稳定状态，但随着 CPB 时间的推移，过滤器上的沉积增加。用伽马相机观察 INN 分布；使用电离室测量总 INN，并使用伽马计数器对纤维和组织样品中的 INN 进行定量。 CPB期间肺中的INN没有显着变化，而肝脏中的INN增加。肺、肝和脑中注射 INN 的百分比随着 CPB 时间的变化而变化，并且与假手术动物相比显着增加。成分的肝素涂层降低了 INN 保留。 3 小时时肺、OX 和 AF 的 INN/米 2 分别为 0.26±0.07%、0.06±0.02% 和 6.17±3.94%，并且在肝素涂层过滤器上显着较低 (2.14±1.30)%。根据猪体内活化 INN 的分布估算内脏和结缔组织的毛细血管表面积（肺，100；肝，134；脾，20；心脏，7；骨骼肌，92；脂肪，12；骨，3；骨髓，5；脑，0.1 米 2）。肺部 INN 保留量远高于 OX/AF 聚合物表面的保留量，表明细胞粘附分子对 INN 在肺和内脏内皮细胞上保留的作用。通过在 CPB 结束时直接连续监测和定量 INN，开发了一种用于 CPB 期间中性粒细胞动力学、边缘化和保留定量的灵敏技术。
Interactions of neutrophils with adsorbed proteins in components of the cardiopulmonary bypass (CPB) circuit and expression of leukocyte adhesion molecules on activated neutrophils affect neutrophil kinetics and margination. Lung and skeletal muscle along with oxygenator (OX) and arterial filter (AF) in the extracorporeal circuit provide the major areas of neutrophil (N) interaction. The dynamics of N-interaction and N-retention during 3 hr CPB was quantified with autologous In-111 labeled neutrophils (INN) in 4 groups of 20 Yorkshire pigs (28–35 kg, 5 sham; 5 CPB, 1 hr; 5 CPB, 3 hr and 5 CPB with heparinized circuit, 3 hr); anesthetized pigs were injected with INN (500–650 µCi), 30 min before CPB and heparinized, and underwent CPB with a roller pump, a hollow fiber OX (Bentley CM 50, 5.0 m 2) and AF (Bentley AF 025, 0.25 m 2) at 2.5–3.6 l/min for 3 hr. N-dynamics on OX and AF was monitored by a calibrated Geiger probe. Neutrophil deposition, like that of plasma proteins on OX, reached a steady state almost instantly, but increased on filter with CPB time. INN distribution was viewed with a gamma camera; total INN was measured with an ion chamber and INN in samples of fibers and tissues was quantified with a gamma counter. INN in lung did not change significantly during CPB and increased in liver. The percentage of injected INN in lung, liver, and brain changed with CPB time and showed significant increase over sham-operated animals. Heparin coating of components decreased INN retention. INN/meter 2 of lung, OX, and AF at 3 hr were 0.26±0.07%, 0.06±0.02%, and 6.17±3.94%, and significantly lower on a heparin coated filter (2.14±1.30)%. Capillary surface areas of viscera and connective tissues (lung, 100; liver, 134; spleen, 20; heart, 7; skeletal muscle, 92; fat, 12; bone, 3; bone marrow, 5; brain, 0.1 meter 2) were estimated from distribution of activated INN in pigs. Lung INN retention was much higher than that of thepolymer surfaces of OX/AF, indicating the role of cell adhesion molecules on INN retention on endothelial cells of lung and viscera. By direct continuous monitoring and quantitation of INN at the end of CPB, a sensitive technique for quantitation of neutrophil kinetics, margination, and retention during CPB was developed.