Purification and characterization of recombinant lipid storage protein-2 from Drosophila melanogaster
Purification and characterization of recombinant lipid storage protein-2 from Drosophila melanogaster
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DOI：
10.2174/092986608785849191
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发表时间：
2008-09-01
影响因子：
1.6
通讯作者：
Soulages, Jose L.
中科院分区：
文献类型：
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作者：
Arrese, Estela L.;Rivera, Laticia;Soulages, Jose L.
脂质储存蛋白2(LSD-2)是一种保守的昆虫蛋白，属于小PAT蛋白家族。PAT蛋白被发现与脂肪细胞的脂滴相关，并在三酰甘油代谢的调节中发挥重要作用。在这里，我们描述了Lsd2的表达和纯化，它在脂蛋白颗粒中的重组，推测的脂结合位点的位置和它的二级结构。本研究为进一步研究LSD2的作用机制提供了起点。讨论了昆虫中仅有的PAT蛋白LSD1和LSD2的异同。
Lipid storage protein 2 (Lsd 2) is a conserved insect protein that belongs to the small PAT family of proteins. PAT proteins are found associated to the lipid droplets of adipocytes and play significant roles in the regulation of triacylglycerides metabolism. Here we describe the expression and purification of Lsd2, its reconstitution in lipoprotein particles, the location of putative lipid binding sites and its secondary structure. This study provides the starting point for future studies on the mechanism of function of Lsd2. The similarities and differences between Lsd1 and Lsd2, the only PAT proteins found in insects, are discussed.