Donor cell differentiation, reprogramming, and cloning efficiency: Elusive or illusive correlation?
Donor cell differentiation, reprogramming, and cloning efficiency: Elusive or illusive correlation?
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DOI：
10.1002/mrd.20654
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发表时间：
2007-05-01
影响因子：
2.5
通讯作者：
Wells, D. N.
中科院分区：
文献类型：
--
作者：
Oback, B.;Wells, D. N.
与其他辅助生殖技术相比，哺乳动物核移植（NT）克隆在产生可存活后代方面效率低下。有人认为，通过选择分化程度较低的细胞类型作为核供体，可以提高核重编程和克隆效率。这一假设主要得到了利用早期卵裂球、胚胎干细胞（ES）细胞和终末分化体细胞供体细胞克隆小鼠的比较实验的支持。我们重新评估了这些比较，考虑到不同的NT程序，来自不同遗传背景、性别、细胞周期阶段的供体细胞的使用，以及在囊胚后发育比较时缺乏可靠的统计意义。我们认为，虽然早期卵裂球的可重编程性似乎比体细胞高得多，但迄今为止还没有最终确定分化状态是否影响体细胞供体细胞的克隆效率。
Compared to other assisted reproductive technologies, mammalian nuclear transfer (NT) cloning is inefficient in generating viable offspring. It has been postulated that nuclear reprogramming and cloning efficiency can be increased by choosing less differentiated cell types as nuclear donors. This hypothesis is mainly supported by comparative mouse cloning experiments using early blastomeres, embryonic stem (ES) cells, and terminally differentiated somatic donor cells. We have re-evaluated these comparisons, taking into account different NT procedures, the use of donor cells from different genetic backgrounds, sex, cell cycle stages, and the lack of robust statistical significance when post-blastocyst development is compared. We argue that while the reprogrammability of early blastomeres appears to be much higher than that of somatic cells, it has so far not been conclusively determined whether differentiation status affects cloning efficiency within somatic donor cell dineages.