Integrative proteomic and microRNA analysis of the priming phase during rat liver regeneration
Integrative proteomic and microRNA analysis of the priming phase during rat liver regeneration
复制标题
大鼠肝脏再生过程中启动阶段的综合蛋白质组学和 microRNA 分析
DOI：
10.1016/j.gene.2015.08.066
复制
发表时间：
2016-01-10
期刊：
影响因子：
3.5
通讯作者：
Xu, Cunshuan
中科院分区：
文献类型：
--
作者：
Geng, Xiaofang;Chang, Cuifang;Xu, Cunshuan
肝部分切除(PH)模型为研究肝再生(LR)提供了一种有效的介质。鉴于LR是由microRNAs(MiRNAs)调控的，研究miRNAs的调控作用对于揭示再生过程是如何启动和控制的至关重要。利用高通量测序技术对大鼠PH后再生肝脏的miRNA表达谱进行检测，发现其中23个miRNA与大鼠LIT相关，其中几个miRNAs在PH后2 h和6 h有明显变化，对应于LR的启动期。此外，我们还利用iTRAQ与LC-MS/MS联用技术检测了PH后2小时和6小时再生大鼠肝脏的蛋白质谱，发现278个蛋白质发生了显著变化。随后，利用Invenity Path Analys9.0(IPA)软件进行的蛋白质组和microRNA综合分析表明，miR-125a、miR-143、miR-150、miR-181c、miR-182、miR-183、miR-199a、miR-429通过调节与细胞凋亡、细胞生存、细胞周期、炎症反应、代谢等关键网络相关的蛋白质的表达，调节大鼠LR的启动阶段。因此，我们的研究为大鼠LR启动阶段由上调的miRNAs下调的靶填充的功能分子网络提供了新的证据。(C)2015爱思唯尔B.V.保留所有权利。
The partial hepatectomy (PH) model provides an effective medium for study of liver regeneration (LR). Considering that LR is regulated by microRNAs (miRNAs), investigation of the regulatory role of miRNAs is critical for revealing how regenerative processes are initiated and controlled. Using high-throughput sequencing technology, we examined miRNA expression profiles of the regenerating rat liver after PH, and found that 23 miRNAs were related to rat Lit Among them, several miRNAs were significantly altered at 2 h and 6 h after PH, corresponding to the priming phase of LR. Furthermore, we examined the protein profiles in the regenerating rat liver at 2 h and 6 h after PH by iTRAQ coupled with LC-MS/MS, and found that 278 proteins were significantly changed. Subsequently, an integrative proteomic and microRNA analysis by Ingenuity Pathway Analysis 9.0 (IPA) software showed that miR-125a, miR-143, miR-150, miR-181c miR-182, miR-183, miR-199a, miR-429 regulated the priming phase of rat LR by modulating the expression of proteins involved in networks critical for cell apoptosis, cell survival, cell cycle, inflammatory response, metabolism, etc. Thus, our studies provide novel evidence for a functional molecular network populated by the down-regulated targets of the up-regulated miRNAs in the priming phase of rat LR. (C) 2015 Elsevier B.V. All rights reserved.