NDR1, A LOCUS OF ARABIDOPSIS-THALIANA THAT IS REQUIRED FOR DISEASE RESISTANCE TO BOTH A BACTERIAL AND A FUNGAL PATHOGEN
NDR1, A LOCUS OF ARABIDOPSIS-THALIANA THAT IS REQUIRED FOR DISEASE RESISTANCE TO BOTH A BACTERIAL AND A FUNGAL PATHOGEN
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DOI：
10.1073/pnas.92.14.6597
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发表时间：
1995-07-03
影响因子：
11.1
通讯作者：
STASKAWICZ, BJ
中科院分区：
文献类型：
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作者：
CENTURY, KS;HOLUB, EB;STASKAWICZ, BJ
我们利用拟南芥作为模型寄主植物，从遗传学角度分析了导致抗病的分子途径，拟南芥加入Col-O对细菌病原体丁香假单胞菌pv和番茄菌株DC3000敏感，但对携带克隆的无毒基因avrB、avrRpm1、avrRpt2和avrPph3中的任何一种DC3000都具有抗性。筛选快中子诱变的Col-O M(2)种子，以鉴定对DC3000（avrB）敏感的突变体。疾病检测和植物细菌生长分析发现了一个突变体ndr1-1（非种族特异性疾病抗性），它对表达四种毒性基因中的任何一种的DC3000敏感。有趣的是，一些菌株仍然引起了过敏样反应。ndr1-1突变还使该植物对几种无毒真菌病原菌Peronospora parasitica敏感。对ndr1-1的遗传分析表明，该突变分离为单隐性位点，位于第3染色体上。ndr1-1突变的特征表明，在对两种不相关病原体的抗性途径中存在一个共同的步骤。
We have employed Arabidopsis thaliana as a model host plant to genetically dissect the molecular pathways leading to disease resistance, A. thaliana accession Col-O is susceptible to the bacterial pathogen Pseudomonas syringae pv, tomato strain DC3000 but resistant in a race-specific manner to DC3000 carrying any one of the cloned avirulence genes avrB, avrRpm1, avrRpt2, and avrPph3. Fast-neutron-mutagenized Col-O M(2) seed was screened to identify mutants susceptible to DC3000(avrB). Disease assays and analysis of in planta bacterial growth identified one mutant, ndr1-1 (non-race-specific disease resistance), that was susceptible to DC3000 expressing any one of the four avirulence genes tested. Interestingly, a hypersensitive-like response was still induced by several of the strains. The ndr1-1 mutation also rendered the plant susceptible to several avirulent isolates of the fungal pathogen Peronospora parasitica. Genetic analysis of ndr1-1 demonstrated that the mutation segregated as a single recessive locus, located on chromosome III. Characterization of the ndr1-1 mutation suggests that a common step exists in pathways of resistance to two unrelated pathogens.