First detection of ungulate tetraparvovirus 1 (bovine hokovirus 1) in domestic yaks in northwestern China
First detection of ungulate tetraparvovirus 1 (bovine hokovirus 1) in domestic yaks in northwestern China
复制标题
中国西北地区首次在家牦牛中检测到有蹄类四细小病毒1型（牛霍科病毒1型）
DOI：
10.1007/s00705-015-2638-1
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发表时间：
2016-01-01
影响因子：
2.7
通讯作者：
Zeng, Qiaoying
中科院分区：
文献类型：
--
作者：
Xu, Fang;Pan, Yangyang;Zeng, Qiaoying
本文报道了在中国西北地区家牦牛（Bos grunniens）中发现的有蹄类四甲状病毒1型（也称为牛hokovirus 1、B-PARV4或四甲状病毒）并进行了系统发育分析。利用保守区对应的B-PARV4引物，采用PCR方法对牦牛血液样本中的B-PARV4基因组进行检测。370份样本中有22份b - parv4相关基因组序列阳性，总体患病率为5.95%。青海省（13/195,6.67%）和甘肃省（9/175,5.14%）的发病率相似，但1岁牦牛（15/177,8.47%）和1岁牦牛（7/193,3.6%）的发病率差异显著（p < 0.05）。对所有22个菌株的近全长基因组序列进行比对，鉴定出6个不同的基因组序列。系统发育分析显示，除JF504698外，这6个基因组序列与所有已知的B-PARV4基因组序列同源性为99.0 ~ 99.7%，JF504698为另一基因型，同源性为88.6%。这是首次在家牦牛中发现b - parv4相关病毒。
We describe the discovery and phylogenetic analysis of ungulate tetraparvovirus 1 (also referred to as bovine hokovirus 1, B-PARV4, or partetravirus) in domestic yaks (Bos grunniens) in northwestern China. The yak B-PARV4 genome was detected in yak blood samples by PCR, using B-PARV4 primers corresponding to conserved regions. Twenty-two of 370 samples were positive for a B-PARV4-related genome sequence, indicating an overall prevalence of 5.95 %. The prevalence in Qinghai Province (13/195, 6.67 %) and Gansu Province (9/175, 5.14 %) was similar, but it varied significantly between yaks a parts per thousand currency sign 1 year old (15/177, 8.47 %) and yaks > 1 year old (7/193, 3.6 %) (p < 0.05). An alignment of the nearly full-length genome sequences of all 22 strains identified six different genomic sequences. A phylogenetic analysis revealed 99.0-99.7 % sequence identity between these six genomes and all known B-PARV4 genomes, excluding JF504698 (only 88.6 % identity), which represents another genotype. This is the first discovery of B-PARV4-related viruses in domestic yaks.