Effect of glutathione on phytochelatin synthesis in tomato cells.
Effect of glutathione on phytochelatin synthesis in tomato cells.
复制标题
谷胱甘肽对番茄细胞中植物螯合素合成的影响。
作者：
M. Mendum;S. Gupta;P. Goldsbrough
番茄、Lycopersicon esculentum Mill 细胞悬浮培养物的生长。 cv VFNT-Cherry 在存在镉的情况下会被丁硫氨酸亚砜亚胺（一种谷胱甘肽合成抑制剂）抑制。通过向培养基中添加谷胱甘肽，用丁硫氨酸亚磺酰亚胺处理的细胞恢复细胞生长和植物螯合素合成。谷胱甘肽刺激镉处理的细胞中植物螯合素的积累，表明谷胱甘肽的可用性可以限制这些肽的合成。外源性谷胱甘肽会导致较小的植物螯合素水平不成比例地增加，特别是[γ-Glu-Cys](2)-Gly。在丁硫氨酸亚磺酰亚胺和谷胱甘肽存在的情况下，暴露于镉时产生的植物螯合素几乎不含有[(35)S]半胱氨酸，这表明这些肽可能不是通过向谷胱甘肽中顺序添加半胱氨酸和谷氨酸来合成的。
Growth of cell suspension cultures of tomato, Lycopersicon esculentum Mill. cv VFNT-Cherry, in the presence of cadmium is inhibited by buthionine sulfoximine, an inhibitor of glutathione synthesis. Cell growth and phytochelatin synthesis are restored to cells treated with buthionine sulfoximine by the addition of glutathione to the medium. Glutathione stimulates the accumulation of phytochelatins in cadmium treated cells, indicating that availability of glutathione can limit synthesis of these peptides. Exogenous glutathione causes a disproportionate increase in the level of smaller phytochelatins, notably [gamma-Glu-Cys](2)-Gly. In the presence of buthionine sulfoximine and glutathione, phytochelatins that are produced upon exposure to cadmium incorporate little [(35)S]cysteine, indicating that these peptides are probably not synthesized by sequential addition of cysteine and glutamate to glutathione.
聚（γ-谷氨酰半胱氨酰）甘氨酸：其在植物细胞耐镉性中的作用。
DOI：
10.1073/pnas.84.19.6619
发表时间：
1987
影响因子：
11.1
作者：
Jackson,PJ;Unkefer,CJ;Doolen,JA;Watt,K;Robinson,NJ
通讯作者：
Robinson,NJ