Characterization of novel circovirus DNAs associated with wasting syndromes in pigs
Characterization of novel circovirus DNAs associated with wasting syndromes in pigs
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DOI：
10.1099/0022-1317-79-9-2171
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发表时间：
1998-09-01
影响因子：
3.8
通讯作者：
Allan, GM
中科院分区：
文献类型：
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作者：
Meehan, BM;McNeilly, F;Allan, GM
猪圆环病毒（PCV）最初被认为是连续猪肾细胞系的污染物，并且不被认为是致病性的。对PCV细胞培养分离株（PCV PK-15）具有反应性的抗体在全球猪群中普遍存在。最近，PCV PK-15样抗原和核酸在北美和欧洲猪的消耗综合征相关病变中得到证实。针对从患有消瘦综合征的猪中分离的圆环病毒产生的单克隆抗体强调了这些圆环病毒与PCV PK-15细胞培养分离株之间的差异。这导致人们猜测，在几个国家的猪群中可能出现了新的致病性PCV。我们报告了从北美和欧洲猪消耗综合征的组织中分离纯化的新型圆环病毒DNA的克隆和特征。这些北美和欧洲的圆环病毒在核苷酸序列水平上形成密切相关的组（>96%组内核苷酸序列同一性），但表现出
Porcine circovirus (PCV) was initially recognized as a contaminant of continuous pig kidney cell lines and was not thought to be pathogenic. Antibodies reactive to the cell culture isolate of PCV (PCV PK-15) are prevalent in the swine population worldwide. Recently, PCV PK-15-like antigen and nucleic acid were demonstrated in lesions associated with wasting syndromes in pigs in North America and Europe. Monoclonal antibodies raised to circoviruses isolated from pigs with wasting syndromes highlighted differences between these circoviruses and the PCV PK-15 cell culture isolate. This has led to speculation that a new pathogenic PCV may have emerged in the swine populations of several countries. We report the cloning and characterization of novel circovirus DNAs purified from virus isolates made from tissues of North American and European pigs with wasting syndromes. These North American and European circoviruses form a closely related group at the nucleotide sequence level (>96% intra-group nucleotide sequence identity) but exhibit