Rapid and high-efficiency generation of mature functional hepatocyte-like cells from adipose-derived stem cells by a three-step protocol.
Rapid and high-efficiency generation of mature functional hepatocyte-like cells from adipose-derived stem cells by a three-step protocol.
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DOI：
10.1186/s13287-015-0181-3
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发表时间：
2015-10-05
影响因子：
7.5
通讯作者：
Deng H
中科院分区：
文献类型：
--
作者：
Xu F;Liu J;Deng J;Chen X;Wang Y;Xu P;Cheng L;Fu Y;Cheng F;Yao Y;Zhang Y;Huang M;Yu D;Wei Y;Deng H
功能性肝细胞的产生是再生医学和药物发现的主要挑战。在这里，我们展示了一种促进在9天内从脂肪源性干细胞（ADSC）生成诱导功能性肝细胞（iHep）的方法。iHep表达肝细胞基因程序并显示成熟肝细胞的特征性功能，包括细胞色素P450酶活性。在移植到具有四氯化碳（CCl 4）诱导的急性暴发性肝功能衰竭的小鼠中后，iHep恢复肝功能并延长存活。这项工作可能有助于开发替代策略，以获得具有生物医学和制药应用潜力的非肝细胞来源的成熟肝细胞。本文的在线版本（doi：10.1186/s13287-015-0181-3）包含补充材料，可供授权用户使用。
The generation of functional hepatocytes is a major challenge for regenerative medicine and drug discovery. Here we show a method that facilitates generation of induced functional hepatocytes (iHeps) from adipose-derived stem cells (ADSCs) within 9 days. iHeps express hepatocytic gene programs and display functions characteristic of mature hepatocytes, including cytochrome P450 enzyme activity. Upon transplantation into mice with carbon tetrachloride (CCl4)-induced acute fulminant liver failure, iHeps restore the liver function and prolong survival. The work could contribute to the development of alternative strategies to obtain nonhepatic cell-derived mature hepatocytes with potential for biomedical and pharmaceutical applications. The online version of this article (doi:10.1186/s13287-015-0181-3) contains supplementary material, which is available to authorized users.