EXPRESSION OF DMRT FAMILY GENES DURING GONADAL DIFFERENTIATION IN TWO SPECIES OF ARTEMIA (BRANCHIOPODA, ANOSTRACA) FROM URMIA LAKE (IRAN)
EXPRESSION OF DMRT FAMILY GENES DURING GONADAL DIFFERENTIATION IN TWO SPECIES OF ARTEMIA (BRANCHIOPODA, ANOSTRACA) FROM URMIA LAKE (IRAN)
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DOI：
10.1163/001121610x527013
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发表时间：
2010-10-01
期刊：
影响因子：
0.6
通讯作者：
Agh, Naser
中科院分区：
文献类型：
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作者：
Farazman, Ali;Inanloo, Kolsoum;Agh, Naser
DMRT（doublemex- and mab-3-related transcription factor）基因家族最初参与果蝇和秀丽隐杆线虫的性别分化，现在已知其参与多种门中物种的早期性别分化。这两个基因在所有生物体中共有的保守基序被称为DM结构域。在本研究中，DMRT相关基因的表达模式的分化性腺的两种水生甲壳动物，卤虫urmiana和卤虫parthenogenetica，这是不同的生殖途径，进行了比较。使用RT-PCR程序对这些物种的无节幼体在早期发育的不同阶段的性腺进行的研究表明，DMRT基因仅在雌性性腺中表达，而不管卤虫的特定物种中普遍存在的繁殖模式。原位杂交在整个安装的性腺在不同的发展阶段证实了我们的RT-PCR结果，这表明，与哺乳动物的情况不同，DMRT基因参与早期卵巢，但不是在睾丸，分化。
The DMRT (doublesex- and mab-3-related transcription factor) family of genes, originally implicated in the sex differentiation of Drosophila and Caenorhabditis elegans, is now known to be involved in early differentiation of sex among species in a variety of phyla. The conserved motif shared by these two genes in all organisms is referred to as the DM domain. In the present study the expression patterns of DMRT-related genes in the differentiating gonads of two species of aquatic crustaceans, Artemia urmiana and Artemia parthenogenetica, which differ in the paths adopted for reproduction, were compared. Studies using the RT-PCR procedure on the gonads of nauplius larvae of these species at different stages of early development indicated that DMRT genes are expressed only in female gonads, regardless of the mode of reproduction prevalent in the specific species of Artemia. In situ hybridization in whole mounts of gonads at different stages of development confirmed our RT-PCR results, suggesting that, unlike the situation in mammals, DMRT genes are involved in the early ovarian, but not in testicular, differentiation.