Purification and characterisation of a non-plant myrosinase from the cabbage aphid Brevicoryne brassicae (L.)
Purification and characterisation of a non-plant myrosinase from the cabbage aphid Brevicoryne brassicae (L.)
复制标题
DOI：
10.1016/s0965-1748(00)00157-0
复制
发表时间：
2001-01-01
影响因子：
3.8
通讯作者：
Rossiter, JT
中科院分区：
文献类型：
--
作者：
Jones, AME;Bridges, M;Rossiter, JT
植物黑芥子酶和芥子油苷是十字花科植物对病虫害的防御系统。首次从甘蓝蚜Brevicoryne brassicae（L.）同质性。蛋白质的N-末端封闭和蛋白酶（胰蛋白酶和赖氨酸C）降解得到的肽，其中5个进行测序。该蛋白质是一个二聚体，亚基分子量为54 kDa+/-500 Da。用抗蚜虫黑芥子酶抗体进行的Western印迹分析表明，与来自芸苔属专家B的蛋白质提取物有很强的交叉反应。芸苔属。抗蚜虫黑芥子酶抗体不与植物黑芥子酶交叉反应，抗植物黑芥子酶抗体也不与蚜虫黑芥子酶交叉反应。(C)2001爱思唯尔科技有限公司版权所有。
Plant myrosinases and glucosinolates constitute a defence system in cruciferous plants towards pests and diseases. We have purified for the first time a non-plant myrosinase from the cabbage aphid Brevicoryne brassicae (L.) to homogeneity. The protein was N-terminally blocked and protease (trypsin and lys c) degradation gave peptides of which five were sequenced. The protein is a dimer with subunits of mass 54 kDa+/-500 Da. Western blot analysis with an anti-aphid myrosinase antibody showed a strong cross reaction with a protein extract from the Brassica specialist, B. brassicae. The anti-aphid myrosinase antibody does not cross react with plant myrosinase neither does an anti-plant myrosinase antibody cross react with aphid myrosinase. (C) 2001 Elsevier Science Ltd. All rights reserved.