Proteomics analysis of the amygdala in rats with CFA-induced pain aversion with electro-acupuncture stimulation
Proteomics analysis of the amygdala in rats with CFA-induced pain aversion with electro-acupuncture stimulation
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电针刺激 CFA 诱导的疼痛厌恶大鼠杏仁核的蛋白质组学分析
DOI：
10.2147/jpr.s211826
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发表时间：
2019-01-01
影响因子：
2.7
通讯作者：
Fang, Jianqiao
中科院分区：
文献类型：
--
作者：
Wu, Yuanyuan;Jiang, Yongliang;Fang, Jianqiao
临床疼痛患者通常表现出对疼痛相关环境的厌恶（疼痛厌恶）。在以往的研究中，电针已被证明是治疗疼痛厌恶的有效方法。杏仁核可能对情绪和疼痛巩固以及一般的疼痛厌恶行为产生重大影响，然而，其潜在机制尚不清楚。目的采用等压标签（Isobaric tags for relative and absolute quanti定量，iTRAQ）方法对完全弗洛伊德佐剂（CFA）诱导的疼痛厌恶大鼠杏仁核进行定量蛋白质组学分析，并对其蛋白表达进行综合分析，探讨CFA对疼痛厌恶的影响机制。材料与方法用100 μL CFA在雄性SD大鼠左后爪足底表面注射诱导炎性疼痛。然后进行cfa诱导的条件厌恶（C-CPA）测试。电刺激双侧足三里、三阴交穴14 d，电刺激频率为2 Hz。基于itraq的蛋白质组学分析，我们研究了杏仁核中的蛋白质表达。结果电刺激可提高毒理学刺激引起的炎性疼痛的足部戒断阈值。在杏仁核中共检测到6319个蛋白。在这些鉴定的蛋白中，疼痛厌恶组与生理盐水组相比鉴定出123个蛋白，疼痛厌恶+ EA组与疼痛厌恶组相比鉴定出125个蛋白显著改变。在疼痛厌恶和ea处理大鼠的杏仁核中发现了11种蛋白质的差异表达。甘油醛-3-磷酸脱氢酶、谷氨酸转运蛋白-1和p21活化激酶6的表达与蛋白质组的结果一致。结论本研究揭示了EA干预疼痛厌恶的中枢神经系统机制。
Background Clinical patients suffering from pain usually exhibit aversion to pain-associated environments (pain aversion). Electro-acupuncture (EA) has been proven to be effective for the treatment of pain aversion in our previous studies. The amygdala could have substantial consequences on emotion and pain consolidation as well as general pain aversion behavior, however, the underlying mechanism remains unclear. Purpose The current study was performed to investigate Isobaric tags for relative and absolute quantitation (iTRAQ) based quantitative proteomic analysis of the amygdala in rats with complete Freund’s adjuvant (CFA)-induced pain aversion, and comprehensive analysis of protein expression were performed to explore the underlying mechanism by which EA affects pain aversion. Materials and methods Inflammatory pain was induced with an intraplantar injection of 100 μL of CFA in the plantar surface of the left hind paw of the male Spragure-Dawley (SD) rats. Then the CFA-induced conditioned place aversion (C-CPA) test was performed. EA stimulation on the bilateral Zusanli and Sanyinjiao acu-points was used for 14 days and the EA stimulation frequency is 2 Hz. Based on iTRAQ-based proteomics analysis, we investigated the protein expression in the amygdala. Results EA can increase the paw withdrawal threshold in inflammatory pain induced by noxious stimulation. A total of 6319 proteins were quantified in amygdala. Of these identified proteins, 123 were identified in the pain aversion group relative to those in the saline group, and 125 significantly altered proteins were identified in the pain aversion + EA group relative to the pain aversion group. A total of 11 proteins were found to be differentially expressed in the amygdala of pain aversion and EA-treated rats. The expression of three proteins, glyceraldehyde-3-phosphate dehydrogenase, glutamate transporter-1, and p21-activated kinase 6, were confirmed to be consistent with the results of the proteome. Conclusion Our investigation demonstrated the possible mechanism of central nerve system by which EA intervetion on pain aversion.