Invasion characteristics of a Plasmodium knowlesi line newly isolated from a human.
Invasion characteristics of a Plasmodium knowlesi line newly isolated from a human.
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DOI：
10.1038/srep24623
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发表时间：
2016-04-21
影响因子：
4.6
通讯作者：
Lau YL
中科院分区：
文献类型：
--
作者：
Amir A;Russell B;Liew JW;Moon RW;Fong MY;Vythilingam I;Subramaniam V;Snounou G;Lau YL
诺氏疟原虫被广泛用作一种重要的疟疾模型，现在被认为是马来西亚人类疟疾的一个重要原因。目前用于研究的诺氏疟原虫菌株是几十年前分离出来的，这引起了人们的担忧，即它们可能不再是当代寄生虫种群的代表。我们从马来西亚吉隆坡收治的一名患者身上获得了一种新的诺氏疟原虫品系（马来亚大学品系，UM01），并将其与从诺氏疟原虫H株中获得的人类适应实验室品系（A1-H.1）进行了比较。UM01和A1-H。1系容易侵入人类和猕猴（Macaca fascicularis）的正常细胞，偏爱网状细胞。尽管两种寄生虫对人类红细胞的入侵依赖于达菲抗原/趋化因子受体（DARC）的存在，但猕猴红细胞的情况并非如此。尽管如此，在入侵效率、配子体产量和无性周期的长度上，两系之间存在差异。为了将来对这种人畜共患物种的实验研究，分离和鉴定大量的诺氏疟原虫系将是明智的。
Plasmodium knowlesi is extensively used as an important malaria model and is now recognized as an important cause of human malaria in Malaysia. The strains of P. knowlesi currently used for research were isolated many decades ago, raising concerns that they might no longer be representative of contemporary parasite populations. We derived a new P. knowlesi line (University Malaya line, UM01), from a patient admitted in Kuala Lumpur, Malaysia, and compared it with a human-adapted laboratory line (A1-H.1) derived from the P. knowlesi H strain. The UM01 and A1-H.1 lines readily invade human and macaque (Macaca fascicularis) normocytes with a preference for reticulocytes. Whereas invasion of human red blood cells was dependent on the presence of the Duffy antigen/receptor for chemokines (DARC) for both parasite lines, this was not the case for macaque red blood cells. Nonetheless, differences in invasion efficiency, gametocyte production and the length of the asexual cycle were noted between the two lines. It would be judicious to isolate and characterise numerous P. knowlesi lines for use in future experimental investigations of this zoonotic species.