Induction of apoptosis in human hepatoma SMMC-7721 cells by solamargine from Solanum nigrum L.
Induction of apoptosis in human hepatoma SMMC-7721 cells by solamargine from Solanum nigrum L.
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DOI：
10.1016/j.jep.2011.11.058
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发表时间：
2012-01-31
影响因子：
5.4
通讯作者：
Gao, Si-Guo
中科院分区：
文献类型：
--
作者：
Ding, Xia;Zhu, Fang-Shi;Gao, Si-Guo
民族药理学相关性：龙葵 L (SNL) 是一种具有利尿、解热和保肝作用的中药，在中国已被用作抗癌治疗偏方的主要成分。 研究目的：本研究的目的是评估从 SNL 中纯化的主要甾体生物碱苷苏拉精 (SM) 对人肝癌 SMMC-7721 的抑制作用材料和方法：采用MU法评估对肿瘤细胞系的IC50。用光学或荧光显微镜观察对形态的影响。使用流式细胞术（FCM）测量细胞凋亡率和细胞周期。比色法检测caspase-3蛋白的表达。结果：SM显着抑制SMMC-7721和HepG2细胞的生长并诱导细胞凋亡。细胞周期分析表明，SM 导致细胞周期停滞在 G2/M 期。而且。 SM可上调caspase-3的表达。结论：本研究表明SM通过激活caspase-3并调节细胞周期进程诱导细胞凋亡并抑制肝癌细胞增殖，在体外对SMMC-7721细胞发挥潜在的抗癌活性。 (C) 2011 Elsevier Ireland Ltd. 保留所有权利。
Ethnopharmacological relevance: Solanum nigrum L (SNL), a traditional Chinese medicinal herb endowed with diuretic, antipyretic and hepatoprotective effects, has been used as a major ingredient of folk prescriptions for anticancer treatment in China.Aim of the study: The purpose of this study was to evaluate the inhibitory effect of solamargine (SM), a major steroidal alkaloid glycoside purified from SNL, on human hepatoma SMMC-7721 cells and investigate the possible mechanism of SM.Materials and methods: The MU assay was used to evaluate the IC50 on tumor cell lines. The effect on morphology was observed with a light or fluorescence microscopy. The rate of apoptosis and the cell cycle were measured using flow cytometry (FCM). The expression of caspase-3 protein was measured by calorimetric assay.Results: SM significantly inhibited the growth of SMMC-7721 and HepG2 cells and induced cell apoptosis. Cell cycle analysis revealed that SM caused cell cycle arrest at the G2/M phase. Moreover. SM could up-regulate the expression of caspase-3.Conclusions: This study indicated that SM exerted potential anticancer activity on SMMC-7721 cells in vitro through the activation of caspase-3 and the regulation of the cell cycle progression to induce apoptosis and inhibit hepatoma cells proliferation. (C) 2011 Elsevier Ireland Ltd. All rights reserved.