Constriction by Dynamin: Elasticity versus Adhesion.
Constriction by Dynamin: Elasticity versus Adhesion.
复制标题
Dynamin 的收缩：弹性与粘附力。
DOI：
10.1016/j.bpj.2016.10.019
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发表时间：
2016
影响因子：
3.4
通讯作者：
M. Deserno
中科院分区：
文献类型：
--
作者：
Z. McDargh;Pablo Vázquez;J. Guven;M. Deserno
当连接几乎分离的膜室的颈部被切断时，任何细胞分裂过程都完成了。这一关键步骤是由发动蛋白家族的蛋白质完成的，它们在这些颈部周围形成螺旋状的螺旋。许多研究都致力于以某种方式阐明这一点的细节，但尽管不乏想法，这个问题并没有解决。图片上明显的扼杀或推动的概念很难用精确的机械术语来表达。此外，由于发动蛋白是一种GT3，因此很容易推测它具有一种运动活动，可以帮助必要的切断动作，但其潜在机制仍然不明显。我们认为，困难是机械上的非平凡性质的限制弹性长丝弯曲的表面，有效的方法来概念化相关的力和扭矩最近才出现。在这里，我们研究了一个概念上简单但在机械上具有挑战性的模型的含义：考虑一个弹性螺旋丝局限于一个表面模仿两个膜室之间的颈部，我们假设采取的形状的悬链。对于这种吸附细丝的预期长度、形状以及它们所施加的力，作为模型中关键参数的函数，我们能说些什么？虽然真实的发动蛋白肯定更复杂，但我们认为这样一个最小模型是必不可少的基线。如果不知道这样一个模型能够解释什么，不能解释什么，就很难证明更复杂的机制是合理的，或者理解这种机制最初是在什么样的约束下发展起来的。
Any cellular fission process is completed when the neck connecting almost-separate membrane compartments is severed. This crucial step is somehow accomplished by proteins from the dynamin family, which polymerize into helical spirals around such necks. Much research has been devoted to elucidating the specifics of that somehow, but despite no shortage of ideas, the question is not settled. Pictorially obvious notions of strangling or pushing are difficult to render in mechanically precise terms. Moreover, because dynamin is a GTPase, it is tempting to speculate that it has a motor activity that assists the necessary severing action, but again the underlying mechanics is not obvious. We believe the difficulty to be the mechanically nontrivial nature of confining elastic filaments onto curved surfaces, for which efficient methods to conceptualize the associated forces and torques have only recently appeared. Here we investigate the implications of a conceptually simple yet mechanically challenging model: consider an elastic helical filament confined to a surface mimicking the neck between two membrane compartments, which we assume to take the shape of a catenoid. What can we say about the expected length of such adsorbed filaments, their shapes, and the forces they exert, as a function of the key parameters in the model? While real dynamin is surely more complex, we consider such a minimal model to be the indispensable baseline. Without knowing what such a model can and cannot explain, it is difficult to justify more complex mechanisms, or understand the constraints under which this machinery evolved in the first place.
影响因子：
3.3
作者：
Yoon, Y;Pitts, KR;McNiven, MA
通讯作者：
McNiven, MA