Galectin-10 mRNA is overexpressed in peripheral blood of aspirin-induced asthma
Galectin-10 mRNA is overexpressed in peripheral blood of aspirin-induced asthma
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DOI：
10.1111/j.1398-9995.2007.01558.x
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发表时间：
2008-01-01
期刊：
影响因子：
12.4
通讯作者：
Pacheco, Y.
中科院分区：
文献类型：
--
作者：
Devouassoux, G.;Pachot, A.;Pacheco, Y.
在敏感患者中，阿司匹林与鼻腔和支气管炎症相关，引起局部症状。尽管该病在临床上有很好的特征，但其生理病理尚不完全清楚，而且缺乏能够有效区分阿司匹林诱导哮喘（AIA）和阿司匹林耐受性哮喘（ATA）的非侵入性方法。该研究的目的是比较AIA和ATA队列的临床特征，并筛选外周血中mRNA表达的差异。服用阿司匹林后出现症状的患者被认为是阿司匹林敏感患者。采集外周血，采用微阵列技术和定量RT-PCR技术定量mRNA表达。数据表明，AIA和ATA在临床表型上有大量相似之处。基因芯片筛选AIA组织中galectin-10 mRNA过表达（AIA/ATA比值= 1.9,P < 0.05）。结果用qRT-PCR证实。微阵列结果与qRT-PCR结果在半乳糖凝集素-10 mRNA表达上呈正相关（r = 0.92, P < 0.0001）。最后，在一组哮喘患者和对照组中验证了qRT-PCR结果，结果显示AIA组与ATA组相比半凝集素-10 mRNA的表达增加（P < 0.001），与对照组相比（P < 0.01）。我们的结果表明，AIA和ATA仍然难以用临床标准区分。采用两种分子生物学方法，我们证明了半乳糖凝集素-10 mRNA在AIA中过表达，这提示了一种新的候选基因和阿司匹林不耐受粘膜炎症的潜在创新途径。
In sensitive patients, aspirin is associated with nasal and bronchial inflammation, eliciting local symptoms. Although the disease is clinically well characterized, its physiopathology is incompletely understood and noninvasive procedures, allowing an effective distinction between aspirin-induced asthma (AIA) and aspirin-tolerant asthma (ATA) are missing.The aims of the study were to compare AIA and ATA cohorts for clinical characteristics and to screen peripheral blood for differential mRNA expression.Patients experiencing symptoms following aspirin ingestion were considered as aspirin sensitive. Peripheral blood was collected to quantify mRNA expression, using microarray technology and quantitative RT-PCR.Data indicated that AIA and ATA share large number of similarities for clinical phenotype. Screening of mRNA expression using microarray showed an overexpression of galectin-10 mRNA in AIA (AIA/ATA ratio = 1.9, P < 0.05). Results were confirmed using qRT-PCR. A positive correlation was established between microarray and qRT-PCR results for galectin-10 mRNA expression (r = 0.92, P < 0.0001). Finally, qRT-PCR results were validated on a subset of asthmatics and controls, showing an increased expression of galectin-10 mRNA in AIA vs ATA (P < 0.001) and vs controls (P < 0.01).Our results demonstrate that AIA and ATA remain difficult to distinguish using clinical criteria. Employing two molecular biological methods, we demonstrate that galectin-10 mRNA is overexpressed in AIA, suggesting a novel candidate gene and a potentially innovative pathway for mucosal inflammation in aspirin intolerance.