Primary and secondary effects of ascorbate on procollagen synthesis and protein synthesis by primary cultures of tendon fibroblasts.
Primary and secondary effects of ascorbate on procollagen synthesis and protein synthesis by primary cultures of tendon fibroblasts.
复制标题
抗坏血酸对肌腱成纤维细胞原代培养物的前胶原合成和蛋白质合成的主要和次要影响。
DOI：
10.1016/0003-9861(76)90301-5
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发表时间：
1976
影响因子：
3.9
通讯作者：
D. Prockop
中科院分区：
文献类型：
--
作者：
W. Kao;J. Flaks;D. Prockop
本实验研究了抗坏血酸对原代培养的鸡胚肌腱成纤维细胞胶原合成的影响。此外，抗坏血酸的文化增加了前胶原羟脯氨酸的合成速率，但效果并没有解释激活脯氨酰羟化酶已在其他细胞培养。相反，羟脯氨酸合成速率的增加似乎是维生素的某些直接辅因子作用的结果。在抗坏血酸的存在下，大多数新合成的前胶原被羟基化，并成为三重螺旋。在抗坏血酸的情况下，在新合成的前胶原的羟基化的整体程度降低，但一小部分的新合成的前胶原是近最大限度的羟基化，并成为三重螺旋。当培养物暴露于抗坏血酸超过6小时，有蛋白质合成率，前胶原合成率，和膜结合核糖体的分数增加。在抗坏血酸的存在下，这些参数的增加似乎是由稳定的三螺旋前胶原在培养系统中的积累所产生的次级效应。
The effects of ascorbic acid on collagen biosynthesis were studied in primary cultures of fibroblasts from chick embryo tendons. Addition of ascorbate to the cultures increased the rate of synthesis of procollagen hydroxyproline, but the effect was not explained by activation of prolyl hydroxylase as has been seen in other cell cultures. Instead the increase in the rate of hydroxyproline synthesis appeared to be the result of some direct cofactor effect of the vitamin. In the presence of ascorbate, most of the newly synthesized procollagen was hydroxylated and became triple helical. In the absence of ascorbate, the overall degree of hydroxylation in newly synthesized procollagen was reduced, but a small fraction of newly synthesized procollagen was near-maximally hydroxylated and became triple helical. When cultures were exposed to ascorbic acid for more than 6 h, there was an increase in rate of protein synthesis, rate of procollagen synthesis, and fraction of membrane-bound ribosomes. The increases in these parameters in the presence of ascorbate appeared to be a secondary effect produced by the accumulation of stable triple-helical procollagen in the culture system.