Inhibition of Staphylococcus adherence to biomaterials by extracellular slime of S. epidermidis RP12.
Inhibition of Staphylococcus adherence to biomaterials by extracellular slime of S. epidermidis RP12.
复制标题
表皮葡萄球菌 RP12 的细胞外粘液抑制葡萄球菌对生物材料的粘附。
DOI：
10.1002/jbm.820281106
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Gristina,AG
中科院分区：
文献类型：
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作者：
Giridhar,G;Kreger,AS;Myrvik,QN;Gristina,AG
在体外研究了凝固酶阴性葡萄球菌的选定菌株对各种生物材料的粘附，以及从表皮葡萄球菌RP 12菌株获得的细胞外粘液对其粘附的抑制。S. epidermidisRP 12比S. hominis和S. hyicus。菌株RP 12对钛合金、超高分子量聚乙烯（UHMWPE）和聚四氟乙烯圆盘的粘附性低于对PMMA圆盘的粘附性。将PMMA盘暴露于从菌株RP 12提取的细胞外粘液大大降低了菌株RP 12、SP 2、SE-360、adnS的粘附。表皮病RP-62 A。活性组分存在于通过Amicon YM 10超滤粗泥获得的<10 kD mol wt级分中;级分的热处理不影响其抑制活性。当细菌和RP 12粘液组分同时加入到PMMA盘中时，>10 kD mol wt的粘液组分竞争性地抑制菌株RP 12对PMMA盘的粘附;相反，<10 kD mol wt的组分增强菌株RP 12对PMMA盘的粘附。John Wiley & Sons，Inc.
Adherence of selected strains of coagulase‐negative staphylococci to various biomaterials, and the inhibition of their adherence by extracellular slime obtained from the RP12 strain ofStaphylococcus epidermidiswere studiedin vitro. S. epidermidisRP12 adhered considerably more to polymethylmethacrylate (PMMA) discs than did the SP2 strain ofS. hominisand the SE‐360 strain ofS. hyicus.Strain RP12 was less adherent to titanium alloy, ultrahigh molecular weight polyethylene (UHMWPE), and Teflon discs than to PMMA discs. Exposure of PMMA discs to extracellular slime extracted from strain RP12 greatly reduced adherence of strain RP12, SP2, SE‐360, adnS. epidermidisRP‐62A. The active component(s) was present in the <10 kD mol wt fraction obtained by Amicon YM10 ultrafiltration of crude slime; heat treatment of the fraction did not affect its inhibitory activity. When the bacteria and RP12 slime fractions were added simultaneously to the PMMA discs, the >10 kD mol wt fraction of slime competitively inhibited adherence of strain RP12 to PMMA discs; in contrast, the <10 kD mol wt fraction enhanced adherence of strain RP12 to PMMA discs. © 1994 John Wiley & Sons, Inc.