Aqueous chromatographic system for the quantification of propofol in biological fluids using a temperature-responsive polymer modified stationary phase.
Aqueous chromatographic system for the quantification of propofol in biological fluids using a temperature-responsive polymer modified stationary phase.
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DOI：
10.1016/j.chroma.2009.04.047
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发表时间：
2009-10
期刊：
影响因子：
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通讯作者：
Tadashi Nishio;Rie Suzuki;Yuko Tsukada;H. Kanazawa;T. Okano;T. Miyabe-Nishiwaki
中科院分区：
文献类型：
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作者：
Tadashi Nishio;Rie Suzuki;Yuko Tsukada;H. Kanazawa;T. Okano;T. Miyabe-Nishiwaki
以n -异丙基丙烯酰胺（NIPAAm）和甲基丙烯酸丁酯（BMA）为固定相，建立了一种用于猴血清丙泊酚（麻醉药）定量分析的新方法。当温度变化触发时，该聚（NIPAAm-co-BMA）共聚物经历了从亲水性到疏水性微观结构的可逆相变。此外，该色谱系统可以通过仅用水作为流动相分离分析物。对80μL血清进行固相萃取预处理，异丙酚和内标回收率均大于77%。该方法的校准范围为0.5μg/mL ~ 10μg/mL，可重复定量给药猴血清中异丙酚的含量。测定内、间相对标准偏差均小于14.1%。此外，该方法的定量值与目前广泛应用的反相高效液相色谱法具有良好的相关性。该方法避免了复杂的流动相制备，且只需改变温度即可调节分析物的保留时间，在临床实践中可用于异丙酚的简单分析。此外，用水代替化石燃料，是绿色化学理想的分析方法。
A new method for the quantitative analysis of monkey serum propofol, which is widely used as an anaesthetic agent, was developed by utilizing a temperature-responsive polymer of N-isopropylacrylamide (NIPAAm) and butyl methacrylate (BMA) as the stationary phase of HPLC–fluorescence detection. This poly(NIPAAm-co-BMA) copolymer undergoes a reversible phase transition from a hydrophilic to a hydrophobic microstructure when triggered by change in the temperature. Also this chromatographic system is possible to separate the analytes by using only water as a mobile phase. A pretreatment of the serum (80μL) was only solid-phase extraction, and the recovery rate of propofol and internal standard was more than 77%, respectively. This method covered the calibration range from 0.5μg/mL to 10μg/mL and allowed a reproducible quantification of the serum propofol in administrated monkey serum. The intra- and inter-assay relative standard deviations were less than 14.1%. In addition, there was good relationship of the quantification values between the developed method and the widely used reversed-phase HPLC method. Our developed method has proven to be useful for a simple analysis of propofol in clinical practice, because the avoidance of complicated mobile phase preparation was possible, and only temperature changing could regulate the retention time of the analyte. In addition, by using water instead of fossil fuel, it is the ideal analytical method according to green chemistry.