A semi-automated method for isolating functionally intact mitochondria from cultured cells and tissue biopsies.

A semi-automated method for isolating functionally intact mitochondria from cultured cells and tissue biopsies.
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一种从培养细胞和组织活检中分离功能完整线粒体的半自动化方法

DOI:
10.1016/j.ab.2013.08.007
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发表时间:
2013
影响因子:
2.9
通讯作者:
Zischka H
Zischka H
中科院分区:
生物学4区
文献类型:
--
作者:
Schmitt S;Saathoff F;Meissner L;Schropp EM;Lichtmannegger J;Schulz S;Eberhagen C;Borchard S;Aichler M;Adamski J;Plesnila N;Rothenfusser S;Kroemer G;Zischka H

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Mitochondrial dysfunctions decisively contribute to the progression of human diseases, implying that functional tests of isolated mitochondria may furnish conclusive information for diagnosis and therapy. Classical mitochondrial isolation methods, however, lack precisely adjustable settings for cell rupture, which is the most critical step in this procedure, and this complicates subsequent analyses. Here, we present an efficient method to isolate functionally active, intact mitochondria from cultured or primary cells and minute tissue samples in a rapid, highly reproducible manner.
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