Enhanced Thermostability and Molecular Insights for L-Asparaginase from Bacillus licheniformis via Structure- and Computation-Based Rational Design
Enhanced Thermostability and Molecular Insights for L-Asparaginase from Bacillus licheniformis via Structure- and Computation-Based Rational Design
复制标题
通过基于结构和计算的理性设计增强地衣芽孢杆菌 L-天冬酰胺酶的热稳定性和分子洞察力
DOI：
10.1021/acs.jafc.2c05712
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发表时间：
2022-11-07
影响因子：
6.1
通讯作者：
Zhu, Ping
中科院分区：
文献类型：
--
作者：
Chi, Huibing;Wang, Yilian;Zhu, Ping
L-Asparaginase has gained much attention for effectively treating acute lymphoblastic leukemia (ALL) and mitigating carcinogenic acrylamide in fried foods. Due to high-dose dependence for clinical treatment and low mitigation efficiency for thermal food processes caused by poor thermal stability, a method to achieve thermostable L-asparaginase has become a critical bottleneck. In this study, a rational design including free energy combined with structural and conservative analyses was applied to engineer the thermostability of L-asparaginase from Bacillus licheniformis (BlAsnase). Two enhanced thermostability mutants D172W and E207A were screened out by site-directed saturation mutagenesis. The double mutant D172W/E207A exhibited highly remarkable thermostability with a 65.8-fold longer half-life at 55 degrees C and 5 degrees C higher optimum reaction temperature and melting temperature (Tm) than those of wild-type BlAsnase. Further, secondary structure, sequence, molecular dynamics (MD), and 3D-structure analysis revealed that the excellent thermostability of the mutant D172W/E207A was on account of increased hydrophobicity and decreased flexibility, highly rigid structure, hydrophobic interactions, and favorable electrostatic potential. As the first report of rationally designing L-asparaginase with improved thermostability from B. licheniformis, this study offers a facile and efficient process to improve the thermostability of L-asparaginase for industrial applications.
