Rapid and simple preparation of mushroom DNA directly from colonies and fruiting bodies for PCR
Rapid and simple preparation of mushroom DNA directly from colonies and fruiting bodies for PCR
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DOI：
10.1007/s10267-012-0182-3
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发表时间：
2012-09-01
期刊：
影响因子：
1.4
通讯作者：
Tanaka, Chihiro
中科院分区：
文献类型：
--
作者：
Izumitsu, Kosuke;Hatoh, Kanako;Tanaka, Chihiro
我们已经优化了一个简单而快速的准备程序蘑菇DNA提取的菌落上的媒体或子实体的PCR扩增。该方法采用微波加热2次，每次1 min，冷却10 min，然后静置5 min，在1 h内可制备100多份蘑菇DNA样品。获得的DNA可用于（1）通过PCR和随后的测序鉴定蘑菇物种，（2）扩增低拷贝数基因（至少2，000bp），和（3）筛选遗传转化体。这项技术将有助于蘑菇物种的真菌学。
We have optimized a simple and rapid preparation procedure for mushroom DNA extraction from colonies on media or from fruiting bodies for PCR amplification. The protocol combines microwaving twice for 1 min, cooling for 10 min, and centrifuging for 5 min. By using this procedure, more than 100 samples of mushroom DNA can be prepared within 1 h. The DNA obtained can be used for (1) identifying mushroom species by PCR and subsequent sequencing, (2) amplifying low copy number genes (at least 2,000 bp), and (3) screening genetic transformants. This technique will contribute to the mycology of mushroom species.