Development and application of a resazurin-based biomass activity test for activated sludge plant management

Development and application of a resazurin-based biomass activity test for activated sludge plant management
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DOI:
10.1016/j.watres.2006.10.002
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发表时间:
2007-01-01
期刊:
影响因子:
12.8
通讯作者:
Quinn, John P.
Quinn, John P.
中科院分区:
环境科学与生态学1区
文献类型:
--
作者:
McNicholl, Bridget P.;McGrath, John W.;Quinn, John P.

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一种快速、可靠且经济有效的测定活性污泥植物生物量代谢活性的方法将成为废水处理行业中有价值的过程控制工具。我们已经开发并优化了一种简单的比色测试方案,该方案基于氧化还原染料reazurin,在新采样的城市污泥和替代活性污泥培养物Polytox中,染料的还原水平与细胞生物量和呼吸速率成正比。该方法已被用于评估贸易废物对两个不同特征的城市活性污泥种群活动的影响。(c) 2006 Elsevier Ltd.版权所有。
A rapid, robust and cost-effective method of assaying the metabolic activity of the biomass of activated sludge plants would be a valuable process control tool in the wastewater treatment industry. We have developed and optimised a simple colorimetric test protocol, based on the redox dye resazurin, in which levels of reduction of the dye are proportional to cell biomass and respiration rate in both freshly sampled municipal sludges and in a surrogate activated sludge culture, Polytox. The method has been used to assess the impact of trade wastes on the activities of two municipal activated sludge populations of differing characteristics. (c) 2006 Elsevier Ltd. All rights reserved.