Identification of Iridoid Glucoside Transporters in Catharanthus roseus.
Identification of Iridoid Glucoside Transporters in Catharanthus roseus.
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DOI：
10.1093/pcp/pcx097
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发表时间：
2017-09-01
影响因子：
4.9
通讯作者：
Halkier BA
中科院分区：
文献类型：
--
作者：
Larsen B;Fuller VL;Pollier J;Van Moerkercke A;Schweizer F;Payne R;Colinas M;O'Connor SE;Goossens A;Halkier BA
吲哚单萜生物碱是一类重要的植物防御化合物和高价值药物。通用MIA前体Secologanin的生物合成在内部韧皮部相关薄壁组织（IPAP）和表皮细胞之间进行。提出的移动的途径中间体的细胞间运输的转运蛋白仍然难以捉摸。筛选在非洲爪蟾卵母细胞中表达的拟南芥转运蛋白文库，鉴定AtNPF 2.9为推定的环烯醚萜苷进口商。在长春花中鉴定出8个直向同源物，其中CrNPF 2.4、CrNPF 2.5和CrNPF 2.6能够将环烯醚萜苷7-脱氧马钱酸、马钱酸、马钱素和裂叶马钱素转运到卵母细胞中。基于酶表达数据和转运蛋白特异性，我们提出，几种酶的生物合成途径中存在于IPAP和表皮细胞，这三个转运蛋白负责运输不仅落干酸，如前所述，但多个中间体。环烯醚萜苷转运CrNPF的鉴定是理解开环环烯醚萜途径复杂协调的重要一步。
Monoterpenoid indole alkaloids (MIAs) are plant defense compounds and high-value pharmaceuticals. Biosynthesis of the universal MIA precursor, secologanin, is organized between internal phloem-associated parenchyma (IPAP) and epidermis cells. Transporters for intercellular transport of proposed mobile pathway intermediates have remained elusive. Screening of an Arabidopsis thaliana transporter library expressed in Xenopus oocytes identified AtNPF2.9 as a putative iridoid glucoside importer. Eight orthologs were identified in Catharanthus roseus, of which three, CrNPF2.4, CrNPF2.5 and CrNPF2.6, were capable of transporting the iridoid glucosides 7-deoxyloganic acid, loganic acid, loganin and secologanin into oocytes. Based on enzyme expression data and transporter specificity, we propose that several enzymes of the biosynthetic pathway are present in both IPAP and epidermis cells, and that the three transporters are responsible for transporting not only loganic acid, as previously proposed, but multiple intermediates. Identification of the iridoid glucoside-transporting CrNPFs is an important step toward understanding the complex orchestration of the seco-iridioid pathway.
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影响因子：
5.3
作者：
Guirimand G;Courdavault V;Lanoue A;Mahroug S;Guihur A;Blanc N;Giglioli-Guivarc'h N;St-Pierre B;Burlat V
通讯作者：
Burlat V
影响因子：
7.2
作者：
Burlat, V;Oudin, A;St-Pierre, B
通讯作者：
St-Pierre, B
影响因子：
7.7
作者：
Jørgensen ME;Xu D;Crocoll C;Ernst HA;Ramírez D;Motawia MS;Olsen CE;Mirza O;Nour-Eldin HH;Halkier BA
通讯作者：
Halkier BA
影响因子：
11.6
作者：
Murata, Jun;Roepke, Jonathon;De Luca, Vincenzo
通讯作者：
De Luca, Vincenzo
影响因子：
4.8
作者：
Ludewig, U;von Wirén, N;Frommer, WB
通讯作者：
Frommer, WB